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Literature summary extracted from

  • Yem, D.W.; Wu, H.C.
    Purification and properties of beta-N-acetylglucosaminidase from Escherichia coli (1976), J. Bacteriol., 125, 324-331.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 Glycopeptide dimeric Escherichia coli

General Stability

EC Number General Stability Organism
3.2.1.52 enzyme unstable, extensive purification only in presence of 20% glycerol possible Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.43
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Escherichia coli
3.2.1.52 2.58
-
 glycopeptide monomer
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 cytoplasm
-
 Escherichia coli 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 33500
-
 gel filtration Escherichia coli
3.2.1.52 36000
-
 1 * 36000, SDS-PAGE Escherichia coli
3.2.1.52 38000
-
 SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Escherichia coli
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 1094fold to near homogeneity Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 162.3
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, in presence of 20% glycerol stable for at least 2 months, even more stable in 50% glycerol Escherichia coli
3.2.1.52 -70°C, in presence of 20% glycerol stable for at least 3 months, even more stable in 50% glycerol Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 glycopeptide dimer + H2O
-
 Escherichia coli N-acetylglucosamine + ?
-
 ?
3.2.1.52 glycopeptide monomer + H2O
-
 Escherichia coli N-acetylglucosamine + ?
-
 ?
3.2.1.52 murein-lipoprotein complex + H2O
-
 Escherichia coli N-acetylglucosamine + ?
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Escherichia coli N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Escherichia coli 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 monomer 1 * 36000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 6.8
-
 crude enzyme preparation Escherichia coli
3.2.1.52 7.7
-
 purified enzyme Escherichia coli