Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kitchen, B.J.; Masters, C.J.
    Purification and properties of bovine mammary gland N-acetyl-beta-D-glucosaminidase (1985), Biochim. Biophys. Acta, 831, 125-132.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.52 medicine veterinary medicine: increased level of enzyme in secreted milk can be detected by diagnosis test for mastitis of the mammary gland of cows Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 extracellular in milk of cows with mastitis of the mammary gland high concentrations of enzymes are found Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 25000
-
 A and B form: 1 * 55000 + 1 * 25000, SDS-PAGE Bos taurus
3.2.1.52 55000
-
 A and B form: 1 * 55000 + 1 * 25000, SDS-PAGE Bos taurus
3.2.1.52 118000
-
 A form, gel filtration Bos taurus
3.2.1.52 220000
-
 B form, sedimentation equilibrium analysis Bos taurus
3.2.1.52 234000
-
 B form, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Bos taurus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein neutral hexose Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 A form, still heterogenous: 63fold, B form, close to homogeneity: 92fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 mammary gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 1.43
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form A Bos taurus
3.2.1.52 2.08
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form B Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
 Bos taurus 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Bos taurus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bos taurus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer A and B form: 1 * 55000 + 1 * 25000, SDS-PAGE Bos taurus