BRENDA - Enzyme Database

Purification and properties of beta-N-acetyl-D-glucosaminidase from alimentary canal of the silkworm, Bombyx mori

Koga, D.; Nakashima, M.; Matsukara, T.; Kimura, S.; Ide, A.; Agric. Biol. Chem. 50, 2357-2368 (1986)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.52
EDTA
0.0075 mM: activity reaches 110.0%
Bombyx mori
General Stability
EC Number
General Stability
Organism
3.2.1.52
lyophilization reduces activity to 34.5%
Bombyx mori
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.52
Ca2+
with 0.03 mM 42.9% of activity remains
Bombyx mori
3.2.1.52
Hg2+
with 0.03 mM 7.1% of activity remains
Bombyx mori
3.2.1.52
Mg2+
with 0.03 mM 71.4% of activity remains
Bombyx mori
3.2.1.52
additional information
high substrate concentrations, i.e. concentrations above 0.2 mM
Bombyx mori
3.2.1.52
Zn2+
with 0.03 mM 88.6% of activity remains
Bombyx mori
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.52
0.056
-
(N-acetyl-beta-D-glucosamine)6
-
Bombyx mori
3.2.1.52
0.09
-
(N-acetyl-beta-D-glucosamine)5
-
Bombyx mori
3.2.1.52
0.118
-
(N-acetyl-beta-D-glucosamine)4
-
Bombyx mori
3.2.1.52
0.122
-
(N-acetyl-beta-D-glucosamine)3
-
Bombyx mori
3.2.1.52
0.397
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Bombyx mori
3.2.1.52
0.433
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
Bombyx mori
3.2.1.52
0.787
-
(N-acetyl-beta-D-glucosamine)2
-
Bombyx mori
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.52
57500
-
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
Ex 1, homodimer: 2 * 67500
Bombyx mori
3.2.1.52
119000
-
gel filtration
Bombyx mori
3.2.1.52
125000
-
SDS-PAGE
Bombyx mori
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
Bombyx mori
-
N-acetyl-D-hexosamines
-
Bombyx mori
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.52
Bombyx mori
-
5-day old pupae
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.52
64fold to homogeneity
Bombyx mori
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
3.2.1.52
hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
exo-type reaction
Bombyx mori
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.52
alimentary canal
-
Bombyx mori
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.52
60.6
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Bombyx mori
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.52
-20°C, after 27 days 87.1% of activity remains
Bombyx mori
3.2.1.52
4°C, not stable, rapid decrease of activity after the first 12 days
Bombyx mori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.52
(N-acetyl-beta-D-glucosamine)2 + H2O
-
393705
Bombyx mori
2 N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)3 + H2O
-
393705
Bombyx mori
(N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)4 + H2O
-
393705
Bombyx mori
(N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)5 + H2O
-
393705
Bombyx mori
(N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)6 + H2O
-
393705
Bombyx mori
(N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
chitin + H2O
colloidal chitin
393705
Bombyx mori
N-acetylglucosamine
-
-
-
?
3.2.1.52
chitin oligosaccharides + H2O
(N-acetyl-D-glucosamine)2-6
393705
Bombyx mori
N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
393705
Bombyx mori
?
-
-
-
?
3.2.1.52
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
393705
Bombyx mori
?
-
-
-
?
3.2.1.52
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
393705
Bombyx mori
2 N,N-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N',N''-triacetylchitotriose + H2O
-
393705
Bombyx mori
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N'-diacetylchitobiose + H2O
-
393705
Bombyx mori
2 N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
-
393705
Bombyx mori
N-acetyl-D-hexosamines
-
393705
Bombyx mori
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
393705
Bombyx mori
2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
393705
Bombyx mori
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
393705
Bombyx mori
2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
393705
Bombyx mori
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.52
dimer
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
dimer
Ex 1, homodimer: 2 * 67500
Bombyx mori
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.52
45
-
alimentary canal enzyme
Bombyx mori
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.52
30
-
stable below, for 10 min
Bombyx mori
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.52
51.9
-
(N-acetyl-beta-D-glucosamine)6
-
Bombyx mori
3.2.1.52
75.6
-
(N-acetyl-beta-D-glucosamine)5
-
Bombyx mori
3.2.1.52
129
-
(N-acetyl-beta-D-glucosamine)4
-
Bombyx mori
3.2.1.52
209
-
(N-acetyl-beta-D-glucosamine)3
-
Bombyx mori
3.2.1.52
820
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Bombyx mori
3.2.1.52
1010
-
(N-acetyl-beta-D-glucosamine)2
-
Bombyx mori
3.2.1.52
1150
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
Bombyx mori
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.52
5.5
6
-
Bombyx mori
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.52
5.5
8.5
stable
Bombyx mori
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.52
EDTA
0.0075 mM: activity reaches 110.0%
Bombyx mori
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.1.52
lyophilization reduces activity to 34.5%
Bombyx mori
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.52
Ca2+
with 0.03 mM 42.9% of activity remains
Bombyx mori
3.2.1.52
Hg2+
with 0.03 mM 7.1% of activity remains
Bombyx mori
3.2.1.52
Mg2+
with 0.03 mM 71.4% of activity remains
Bombyx mori
3.2.1.52
additional information
high substrate concentrations, i.e. concentrations above 0.2 mM
Bombyx mori
3.2.1.52
Zn2+
with 0.03 mM 88.6% of activity remains
Bombyx mori
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.52
0.056
-
(N-acetyl-beta-D-glucosamine)6
-
Bombyx mori
3.2.1.52
0.09
-
(N-acetyl-beta-D-glucosamine)5
-
Bombyx mori
3.2.1.52
0.118
-
(N-acetyl-beta-D-glucosamine)4
-
Bombyx mori
3.2.1.52
0.122
-
(N-acetyl-beta-D-glucosamine)3
-
Bombyx mori
3.2.1.52
0.397
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Bombyx mori
3.2.1.52
0.433
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
Bombyx mori
3.2.1.52
0.787
-
(N-acetyl-beta-D-glucosamine)2
-
Bombyx mori
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.52
57500
-
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
Ex 1, homodimer: 2 * 67500
Bombyx mori
3.2.1.52
119000
-
gel filtration
Bombyx mori
3.2.1.52
125000
-
SDS-PAGE
Bombyx mori
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
Bombyx mori
-
N-acetyl-D-hexosamines
-
Bombyx mori
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.52
64fold to homogeneity
Bombyx mori
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.52
alimentary canal
-
Bombyx mori
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.52
60.6
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Bombyx mori
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.52
-20°C, after 27 days 87.1% of activity remains
Bombyx mori
3.2.1.52
4°C, not stable, rapid decrease of activity after the first 12 days
Bombyx mori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.52
(N-acetyl-beta-D-glucosamine)2 + H2O
-
393705
Bombyx mori
2 N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)3 + H2O
-
393705
Bombyx mori
(N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)4 + H2O
-
393705
Bombyx mori
(N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)5 + H2O
-
393705
Bombyx mori
(N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)6 + H2O
-
393705
Bombyx mori
(N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
chitin + H2O
colloidal chitin
393705
Bombyx mori
N-acetylglucosamine
-
-
-
?
3.2.1.52
chitin oligosaccharides + H2O
(N-acetyl-D-glucosamine)2-6
393705
Bombyx mori
N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
393705
Bombyx mori
?
-
-
-
?
3.2.1.52
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
393705
Bombyx mori
?
-
-
-
?
3.2.1.52
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
393705
Bombyx mori
2 N,N-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N',N''-triacetylchitotriose + H2O
-
393705
Bombyx mori
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N'-diacetylchitobiose + H2O
-
393705
Bombyx mori
2 N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
-
393705
Bombyx mori
N-acetyl-D-hexosamines
-
393705
Bombyx mori
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
393705
Bombyx mori
2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
393705
Bombyx mori
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
393705
Bombyx mori
2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
393705
Bombyx mori
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.52
dimer
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
dimer
Ex 1, homodimer: 2 * 67500
Bombyx mori
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.52
45
-
alimentary canal enzyme
Bombyx mori
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.52
30
-
stable below, for 10 min
Bombyx mori
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.52
51.9
-
(N-acetyl-beta-D-glucosamine)6
-
Bombyx mori
3.2.1.52
75.6
-
(N-acetyl-beta-D-glucosamine)5
-
Bombyx mori
3.2.1.52
129
-
(N-acetyl-beta-D-glucosamine)4
-
Bombyx mori
3.2.1.52
209
-
(N-acetyl-beta-D-glucosamine)3
-
Bombyx mori
3.2.1.52
820
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Bombyx mori
3.2.1.52
1010
-
(N-acetyl-beta-D-glucosamine)2
-
Bombyx mori
3.2.1.52
1150
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
Bombyx mori
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.52
5.5
6
-
Bombyx mori
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.52
5.5
8.5
stable
Bombyx mori