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Literature summary extracted from

  • Koga, D.; Nakashima, M.; Matsukara, T.; Kimura, S.; Ide, A.
    Purification and properties of beta-N-acetyl-D-glucosaminidase from alimentary canal of the silkworm, Bombyx mori (1986), Agric. Biol. Chem., 50, 2357-2368.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 EDTA 0.0075 mM: activity reaches 110.0% Bombyx mori

General Stability

EC Number General Stability Organism
3.2.1.52 lyophilization reduces activity to 34.5% Bombyx mori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ca2+ with 0.03 mM 42.9% of activity remains Bombyx mori
3.2.1.52 Hg2+ with 0.03 mM 7.1% of activity remains Bombyx mori
3.2.1.52 Mg2+ with 0.03 mM 71.4% of activity remains Bombyx mori
3.2.1.52 additional information high substrate concentrations, i.e. concentrations above 0.2 mM Bombyx mori
3.2.1.52 Zn2+ with 0.03 mM 88.6% of activity remains Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.056
-
 (N-acetyl-beta-D-glucosamine)6
-
 Bombyx mori
3.2.1.52 0.09
-
 (N-acetyl-beta-D-glucosamine)5
-
 Bombyx mori
3.2.1.52 0.118
-
 (N-acetyl-beta-D-glucosamine)4
-
 Bombyx mori
3.2.1.52 0.122
-
 (N-acetyl-beta-D-glucosamine)3
-
 Bombyx mori
3.2.1.52 0.397
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Bombyx mori
3.2.1.52 0.433
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Bombyx mori
3.2.1.52 0.787
-
 (N-acetyl-beta-D-glucosamine)2
-
 Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 57500
-
 alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
3.2.1.52 67500
-
 alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
3.2.1.52 67500
-
 Ex 1, homodimer: 2 * 67500 Bombyx mori
3.2.1.52 119000
-
 gel filtration Bombyx mori
3.2.1.52 125000
-
 SDS-PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Bombyx mori
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bombyx mori
-
 5-day old pupae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 64fold to homogeneity Bombyx mori

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Bombyx mori
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 alimentary canal
-
 Bombyx mori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 60.6
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Bombyx mori

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, after 27 days 87.1% of activity remains Bombyx mori
3.2.1.52 4°C, not stable, rapid decrease of activity after the first 12 days Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 (N-acetyl-beta-D-glucosamine)2 + H2O
-
 Bombyx mori 2 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)3 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)4 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)5 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 (N-acetyl-beta-D-glucosamine)6 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 chitin + H2O colloidal chitin Bombyx mori N-acetylglucosamine
-
 ?
3.2.1.52 chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-6 Bombyx mori N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Bombyx mori ?
-
 ?
3.2.1.52 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Bombyx mori ?
-
 ?
3.2.1.52 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Bombyx mori 2 N,N-diacetylchitobiose
-
 ?
3.2.1.52 N,N',N''-triacetylchitotriose + H2O
-
 Bombyx mori N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Bombyx mori deacetylated chitooligosaccharides + acetate
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Bombyx mori N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Bombyx mori 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bombyx mori 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
3.2.1.52 dimer Ex 1, homodimer: 2 * 67500 Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 45
-
 alimentary canal enzyme Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 30
-
 stable below, for 10 min Bombyx mori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 51.9
-
 (N-acetyl-beta-D-glucosamine)6
-
 Bombyx mori
3.2.1.52 75.6
-
 (N-acetyl-beta-D-glucosamine)5
-
 Bombyx mori
3.2.1.52 129
-
 (N-acetyl-beta-D-glucosamine)4
-
 Bombyx mori
3.2.1.52 209
-
 (N-acetyl-beta-D-glucosamine)3
-
 Bombyx mori
3.2.1.52 820
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Bombyx mori
3.2.1.52 1010
-
 (N-acetyl-beta-D-glucosamine)2
-
 Bombyx mori
3.2.1.52 1150
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5.5 6
-
 Bombyx mori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 5.5 8.5 stable Bombyx mori