BRENDA - Enzyme Database

beta-N-Acetyl-D-glucosaminidases from integument of the silkworm, Bombyx mori: Comparative biochemistry with the pupal alimentary canal enzyme

Koga, D.; Shimazaki, C.; Yamamoto, K.; Inoue, K.; Kimura, S.; Ide, A.; Agric. Biol. Chem. 51, 1679-1681 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.52
Hg2+
Ex 1, Ex 2 and alimentary canal enzyme
Bombyx mori
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.52
0.03
-
(N-acetyl-beta-D-glucosamine)4
Ex 2
Bombyx mori
3.2.1.52
0.05
-
(N-acetyl-beta-D-glucosamine)3
Ex 2
Bombyx mori
3.2.1.52
0.06
-
(N-acetyl-beta-D-glucosamine)6
Ex 2
Bombyx mori
3.2.1.52
0.07
-
(N-acetyl-beta-D-glucosamine)5
Ex 1
Bombyx mori
3.2.1.52
0.09
-
(N-acetyl-beta-D-glucosamine)5
Ex 2
Bombyx mori
3.2.1.52
0.1
-
(N-acetyl-beta-D-glucosamine)6
Ex 1
Bombyx mori
3.2.1.52
0.16
-
(N-acetyl-beta-D-glucosamine)4
Ex 1
Bombyx mori
3.2.1.52
0.31
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
Ex 2
Bombyx mori
3.2.1.52
0.42
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Ex 2
Bombyx mori
3.2.1.52
0.49
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Ex 1
Bombyx mori
3.2.1.52
0.64
-
(N-acetyl-beta-D-glucosamine)2
Ex 2
Bombyx mori
3.2.1.52
0.64
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
Ex 1
Bombyx mori
3.2.1.52
0.67
-
(N-acetyl-beta-D-glucosamine)2
Ex 1
Bombyx mori
3.2.1.52
0.87
-
(N-acetyl-beta-D-glucosamine)3
Ex 1
Bombyx mori
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.52
57500
-
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
57500
-
Ex 2: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
Ex 2: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
Ex 1, homodimer: 2 * 67500
Bombyx mori
3.2.1.52
125000
-
Ex 2 and alimentary canal enzyme, gel filtration
Bombyx mori
3.2.1.52
135000
-
Ex 1, gel filtration
Bombyx mori
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
Bombyx mori
-
N-acetyl-D-hexosamines
-
Bombyx mori
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.52
Bombyx mori
-
silkworm, pupae and fifth-instar larvae at the SP3 stage
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.52
Ex 1: 3000fold, Ex 2: 3200fold
Bombyx mori
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
3.2.1.52
hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
exo-type reaction
Bombyx mori
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.52
alimentary canal
-
Bombyx mori
-
3.2.1.52
integument
-
Bombyx mori
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.52
(N-acetyl-beta-D-glucosamine)2 + H2O
-
393704
Bombyx mori
2 N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)3 + H2O
-
393704
Bombyx mori
(N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)4 + H2O
-
393704
Bombyx mori
(N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)5 + H2O
-
393704
Bombyx mori
(N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)6 + H2O
-
393704
Bombyx mori
(N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
chitin oligosaccharides + H2O
(N-acetyl-D-glucosamine)2-6
393704
Bombyx mori
N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
393704
Bombyx mori
?
-
-
-
?
3.2.1.52
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
393704
Bombyx mori
?
-
-
-
?
3.2.1.52
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
393704
Bombyx mori
2 N,N-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N',N''-triacetylchitotriose + H2O
-
393704
Bombyx mori
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N'-diacetylchitobiose + H2O
-
393704
Bombyx mori
2 N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
-
393704
Bombyx mori
N-acetyl-D-hexosamines
-
393704
Bombyx mori
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
393704
Bombyx mori
2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
393704
Bombyx mori
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
393704
Bombyx mori
2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
393704
Bombyx mori
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.52
dimer
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
dimer
Ex 2: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
dimer
Ex 1, homodimer: 2 * 67500
Bombyx mori
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.52
45
-
alimentary canal enzyme
Bombyx mori
3.2.1.52
55
-
Ex 1
Bombyx mori
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.52
40
-
alimentary canal enzyme, stable below
Bombyx mori
3.2.1.52
48
-
Ex 1, stable below
Bombyx mori
3.2.1.52
58
-
alimentary canal enzyme, 50% of activity remains
Bombyx mori
3.2.1.52
62
-
Ex 1, 50% of activity remains
Bombyx mori
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.52
29
-
(N-acetyl-beta-D-glucosamine)6
Ex 2
Bombyx mori
3.2.1.52
42
-
(N-acetyl-beta-D-glucosamine)4
Ex 2
Bombyx mori
3.2.1.52
55
-
(N-acetyl-beta-D-glucosamine)6
Ex 1
Bombyx mori
3.2.1.52
57
-
(N-acetyl-beta-D-glucosamine)5
Ex 1
Bombyx mori
3.2.1.52
76
-
(N-acetyl-beta-D-glucosamine)5
Ex 2
Bombyx mori
3.2.1.52
82
-
(N-acetyl-beta-D-glucosamine)3
Ex 2
Bombyx mori
3.2.1.52
110
-
(N-acetyl-beta-D-glucosamine)4
Ex 1
Bombyx mori
3.2.1.52
130
-
(N-acetyl-beta-D-glucosamine)3
Ex 1
Bombyx mori
3.2.1.52
320
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
Ex 2
Bombyx mori
3.2.1.52
570
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
Ex 1
Bombyx mori
3.2.1.52
580
-
(N-acetyl-beta-D-glucosamine)2
Ex 2
Bombyx mori
3.2.1.52
620
-
(N-acetyl-beta-D-glucosamine)2
Ex 1
Bombyx mori
3.2.1.52
720
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Ex 2
Bombyx mori
3.2.1.52
890
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Ex 1
Bombyx mori
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.52
5
6
Ex 1, Ex 2 and alimentary canal enzyme
Bombyx mori
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.52
5
9
Ex 2, stable
Bombyx mori
3.2.1.52
5
8.5
alimentary canal enzyme, stable
Bombyx mori
3.2.1.52
6
8
Ex 1, stable
Bombyx mori
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.52
Hg2+
Ex 1, Ex 2 and alimentary canal enzyme
Bombyx mori
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.52
0.03
-
(N-acetyl-beta-D-glucosamine)4
Ex 2
Bombyx mori
3.2.1.52
0.05
-
(N-acetyl-beta-D-glucosamine)3
Ex 2
Bombyx mori
3.2.1.52
0.06
-
(N-acetyl-beta-D-glucosamine)6
Ex 2
Bombyx mori
3.2.1.52
0.07
-
(N-acetyl-beta-D-glucosamine)5
Ex 1
Bombyx mori
3.2.1.52
0.09
-
(N-acetyl-beta-D-glucosamine)5
Ex 2
Bombyx mori
3.2.1.52
0.1
-
(N-acetyl-beta-D-glucosamine)6
Ex 1
Bombyx mori
3.2.1.52
0.16
-
(N-acetyl-beta-D-glucosamine)4
Ex 1
Bombyx mori
3.2.1.52
0.31
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
Ex 2
Bombyx mori
3.2.1.52
0.42
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Ex 2
Bombyx mori
3.2.1.52
0.49
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Ex 1
Bombyx mori
3.2.1.52
0.64
-
(N-acetyl-beta-D-glucosamine)2
Ex 2
Bombyx mori
3.2.1.52
0.64
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
Ex 1
Bombyx mori
3.2.1.52
0.67
-
(N-acetyl-beta-D-glucosamine)2
Ex 1
Bombyx mori
3.2.1.52
0.87
-
(N-acetyl-beta-D-glucosamine)3
Ex 1
Bombyx mori
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.52
57500
-
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
57500
-
Ex 2: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
Ex 2: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
67500
-
Ex 1, homodimer: 2 * 67500
Bombyx mori
3.2.1.52
125000
-
Ex 2 and alimentary canal enzyme, gel filtration
Bombyx mori
3.2.1.52
135000
-
Ex 1, gel filtration
Bombyx mori
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
Bombyx mori
-
N-acetyl-D-hexosamines
-
Bombyx mori
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.52
Ex 1: 3000fold, Ex 2: 3200fold
Bombyx mori
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.52
alimentary canal
-
Bombyx mori
-
3.2.1.52
integument
-
Bombyx mori
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.52
(N-acetyl-beta-D-glucosamine)2 + H2O
-
393704
Bombyx mori
2 N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)3 + H2O
-
393704
Bombyx mori
(N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)4 + H2O
-
393704
Bombyx mori
(N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)5 + H2O
-
393704
Bombyx mori
(N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
(N-acetyl-beta-D-glucosamine)6 + H2O
-
393704
Bombyx mori
(N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
-
-
?
3.2.1.52
chitin oligosaccharides + H2O
(N-acetyl-D-glucosamine)2-6
393704
Bombyx mori
N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
393704
Bombyx mori
?
-
-
-
?
3.2.1.52
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
393704
Bombyx mori
?
-
-
-
?
3.2.1.52
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
393704
Bombyx mori
2 N,N-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N',N''-triacetylchitotriose + H2O
-
393704
Bombyx mori
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
3.2.1.52
N,N'-diacetylchitobiose + H2O
-
393704
Bombyx mori
2 N-acetyl-D-glucosamine
-
-
-
?
3.2.1.52
N-acetyl-beta-D-hexosaminides + H2O
-
393704
Bombyx mori
N-acetyl-D-hexosamines
-
393704
Bombyx mori
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
393704
Bombyx mori
2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
393704
Bombyx mori
?
3.2.1.52
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
393704
Bombyx mori
2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
393704
Bombyx mori
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.52
dimer
alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
dimer
Ex 2: 1 * 67500 + 1 * 57500, SDS-PAGE
Bombyx mori
3.2.1.52
dimer
Ex 1, homodimer: 2 * 67500
Bombyx mori
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.52
45
-
alimentary canal enzyme
Bombyx mori
3.2.1.52
55
-
Ex 1
Bombyx mori
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.52
40
-
alimentary canal enzyme, stable below
Bombyx mori
3.2.1.52
48
-
Ex 1, stable below
Bombyx mori
3.2.1.52
58
-
alimentary canal enzyme, 50% of activity remains
Bombyx mori
3.2.1.52
62
-
Ex 1, 50% of activity remains
Bombyx mori
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.52
29
-
(N-acetyl-beta-D-glucosamine)6
Ex 2
Bombyx mori
3.2.1.52
42
-
(N-acetyl-beta-D-glucosamine)4
Ex 2
Bombyx mori
3.2.1.52
55
-
(N-acetyl-beta-D-glucosamine)6
Ex 1
Bombyx mori
3.2.1.52
57
-
(N-acetyl-beta-D-glucosamine)5
Ex 1
Bombyx mori
3.2.1.52
76
-
(N-acetyl-beta-D-glucosamine)5
Ex 2
Bombyx mori
3.2.1.52
82
-
(N-acetyl-beta-D-glucosamine)3
Ex 2
Bombyx mori
3.2.1.52
110
-
(N-acetyl-beta-D-glucosamine)4
Ex 1
Bombyx mori
3.2.1.52
130
-
(N-acetyl-beta-D-glucosamine)3
Ex 1
Bombyx mori
3.2.1.52
320
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
Ex 2
Bombyx mori
3.2.1.52
570
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
Ex 1
Bombyx mori
3.2.1.52
580
-
(N-acetyl-beta-D-glucosamine)2
Ex 2
Bombyx mori
3.2.1.52
620
-
(N-acetyl-beta-D-glucosamine)2
Ex 1
Bombyx mori
3.2.1.52
720
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Ex 2
Bombyx mori
3.2.1.52
890
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Ex 1
Bombyx mori
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.52
5
6
Ex 1, Ex 2 and alimentary canal enzyme
Bombyx mori
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.52
5
9
Ex 2, stable
Bombyx mori
3.2.1.52
5
8.5
alimentary canal enzyme, stable
Bombyx mori
3.2.1.52
6
8
Ex 1, stable
Bombyx mori