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Literature summary extracted from

  • Pocsi, I.; Kiss, L.; Zsoldos-Madi, V.; Pinter, I.
    Studies on the N-acetyl-beta-D-hexosaminidase B from germinating Lupinus luteus L. seeds: II. Mechanism and inhibition with some 2-acetamido-2-deoxyaldono(1-4)lactones (1990), Biochim. Biophys. Acta, 1039, 119-122.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 2-acetamido-2-deoxy-D-fucono(1-4)lactone
-
 Lupinus luteus
3.2.1.52 2-acetamido-2-deoxy-D-galactono(1-4)lactone
-
 Lupinus luteus
3.2.1.52 2-acetamido-2-deoxy-L-arabinono(1-4)lactone
-
 Lupinus luteus
3.2.1.52 2-acetamido-6-azido-2,6-dideoxy-D-galactono(1-4)lactone
-
 Lupinus luteus
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Lupinus luteus
3.2.1.52 thiophenyl-N-acetyl-beta-D-glucosaminide
-
 Lupinus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.58
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Lupinus luteus
3.2.1.52 0.83
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Lupinus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Lupinus luteus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Lupinus luteus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.52 (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose same active site for N-glucosaminidase and N-galactosaminidase activities Lupinus luteus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 seed germinating seeds Lupinus luteus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Lupinus luteus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Lupinus luteus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Lupinus luteus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?