EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.52 | bovine serum albumin | at 5% both hexosaminidase activities of forms II and I are increased 2- and 1.5fold | Trigonella foenum-graecum | |
3.2.1.52 | Fe2+ | stimulates both hexosaminidase activities of form I | Trigonella foenum-graecum | |
3.2.1.52 | Fe3+ | stimulates galactosaminidase activitiy of form I | Trigonella foenum-graecum | |
3.2.1.52 | L-cysteine | concentrations of 0.05, 0.1, 0.4 and 0.7 mM are needed to reactivate both hexosaminidase activities of forms I, II, III and IV | Trigonella foenum-graecum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.52 | Ag+ | all 4 forms | Trigonella foenum-graecum | |
3.2.1.52 | Fe2+ | both hexosaminidase activities of forms II, III and IV inhibited | Trigonella foenum-graecum | |
3.2.1.52 | Fe3+ | glucosaminidase-activity of forms II, III and IV are depressed, galactosaminidase-activity of forms III and IV are inhibited | Trigonella foenum-graecum | |
3.2.1.52 | Hg2+ | all 4 forms | Trigonella foenum-graecum | |
3.2.1.52 | p-chloromercuribenzoate | inhibits forms II, III and IV, competitive inhibition | Trigonella foenum-graecum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.52 | additional information | - |
additional information | KM 0.056, new form I, after 4 months of storage at 4°C | Trigonella foenum-graecum | |
3.2.1.52 | 0.04 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | form I | Trigonella foenum-graecum | |
3.2.1.52 | 0.04 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | form IV | Trigonella foenum-graecum | |
3.2.1.52 | 0.087 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | form III | Trigonella foenum-graecum | |
3.2.1.52 | 0.09 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | form III | Trigonella foenum-graecum | |
3.2.1.52 | 0.15 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | form I | Trigonella foenum-graecum | |
3.2.1.52 | 0.37 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | form IV | Trigonella foenum-graecum | |
3.2.1.52 | 0.55 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | form II | Trigonella foenum-graecum | |
3.2.1.52 | 0.62 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | form II | Trigonella foenum-graecum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 24000 | - |
form II, SDS-PAGE | Trigonella foenum-graecum |
3.2.1.52 | 28000 | - |
form I, SDS-PAGE | Trigonella foenum-graecum |
3.2.1.52 | 30000 | - |
form IV, SDS-PAGE | Trigonella foenum-graecum |
3.2.1.52 | 30500 | - |
form III, SDS-PAGE | Trigonella foenum-graecum |
3.2.1.52 | 62000 | - |
new form I, after 4 months of storage at 4°C | Trigonella foenum-graecum |
3.2.1.52 | 72000 | - |
form II, gel filtration | Trigonella foenum-graecum |
3.2.1.52 | 84000 | - |
form I, gel filtration | Trigonella foenum-graecum |
3.2.1.52 | 150000 | - |
form IV, gel filtration | Trigonella foenum-graecum |
3.2.1.52 | 180000 | - |
form III, gel filtration | Trigonella foenum-graecum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | Trigonella foenum-graecum | - |
N-acetyl-D-hexosamines | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.52 | Trigonella foenum-graecum | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.52 | glycoprotein | mannosylation | Trigonella foenum-graecum |
3.2.1.52 | glycoprotein | N-acetyl-glucosaminylation | Trigonella foenum-graecum |
3.2.1.52 | glycoprotein | fucosylation | Trigonella foenum-graecum |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.52 | form I: 2262fold, form II: 2102fold, form III: 20380fold, form IV: 5061fold | Trigonella foenum-graecum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.52 | cotyledon | forms I and II | Trigonella foenum-graecum | - |
3.2.1.52 | embryo | forms III and IV | Trigonella foenum-graecum | - |
3.2.1.52 | endosperm | forms I and II | Trigonella foenum-graecum | - |
3.2.1.52 | seed | germinating seeds | Trigonella foenum-graecum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 2.64 | - |
form II | Trigonella foenum-graecum |
3.2.1.52 | 2.82 | - |
form I | Trigonella foenum-graecum |
3.2.1.52 | 6.35 | - |
form IV | Trigonella foenum-graecum |
3.2.1.52 | 25.68 | - |
form III | Trigonella foenum-graecum |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.52 | 4°C, after 2 months of storage in phosphate buffer pH 5.0 only form I shows decrease of activity by 50-60%, after 4 months a new form of enzyme with a molecular weight of 62000 Da and a Km of 0.056 mM appears | Trigonella foenum-graecum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.52 | 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O | - |
Trigonella foenum-graecum | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside | - |
? | |
3.2.1.52 | 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Trigonella foenum-graecum | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
3.2.1.52 | additional information | - |
Trigonella foenum-graecum | ? | - |
? | |
3.2.1.52 | N-acetyl-beta-D-hexosaminides + H2O | - |
Trigonella foenum-graecum | N-acetyl-D-hexosamines | - |
? | |
3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Trigonella foenum-graecum | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
3.2.1.52 | p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Trigonella foenum-graecum | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.52 | hexamer | 6 * 30500, SDS-PAGE, form III | Trigonella foenum-graecum |
3.2.1.52 | pentamer | 5 * 30000, SDS-PAGE, form IV | Trigonella foenum-graecum |
3.2.1.52 | trimer | 3 * 28000, SDS-PAGE, form I | Trigonella foenum-graecum |
3.2.1.52 | trimer | 3 * 24000, SDS-PAGE, form II | Trigonella foenum-graecum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 40 | 45 | all 4 forms | Trigonella foenum-graecum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 37 | - |
at its optimum pH both hexosaminidase activities of each of the 4 forms remain stable over 5 hours | Trigonella foenum-graecum |
3.2.1.52 | 50 | - |
both hexosaminidase activities of form I decrease rapidly, forms II, III and IV show biphasic denaturation curves | Trigonella foenum-graecum |
3.2.1.52 | 60 | - |
complete inactivation of all 4 forms after 20 min | Trigonella foenum-graecum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 4.4 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form III | Trigonella foenum-graecum |
3.2.1.52 | 4.6 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form IV | Trigonella foenum-graecum |
3.2.1.52 | 4.8 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form IV | Trigonella foenum-graecum |
3.2.1.52 | 5 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, forms II and III | Trigonella foenum-graecum |
3.2.1.52 | 5 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form II | Trigonella foenum-graecum |
3.2.1.52 | 5.5 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form I | Trigonella foenum-graecum |
3.2.1.52 | 6 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form I | Trigonella foenum-graecum |