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Literature summary extracted from

  • Yamamoto, K.; Tsuji, Y.; Matsushita, S.; Kumagai, H.; Tochikura, T.
    Purification and properties of beta-N-acetylhexosaminidase from Mucor fragilis grown in bovine blood (1986), Appl. Environ. Microbiol., 51, 1019-1023.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Hg2+ low molecular weight form EII, at 0.25 mM Mucor fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.28
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside low molecular weight form EII Mucor fragilis
3.2.1.52 0.46
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside low molecular weight form EII Mucor fragilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 extracellular
-
 Mucor fragilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 70000
-
 low molecular weight form EII, SDS-PAGE Mucor fragilis
3.2.1.52 125000
-
 low molecular weight form EII, gel filtration Mucor fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Mucor fragilis
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Mucor fragilis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.52 glycoprotein low molecular weight form EII Mucor fragilis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 low molecular weight form EII, 3500fold to homogeneity Mucor fragilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 culture filtrate low molecular weight form Mucor fragilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 14.2
-
 low molecular weight form EII, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Mucor fragilis
3.2.1.52 35.2
-
 low molecular weight form EII, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Mucor fragilis

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, low molecular weight form EII, concentrated by ultrafiltration Mucor fragilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 glycopeptide + H2O low molecular weight form EII Mucor fragilis N-acetyl-D-glucosamine
-
 ?
3.2.1.52 glycopeptide + H2O glycopeptide obtained by modification of fetuin Mucor fragilis N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O low molecular weight form EII Mucor fragilis N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Mucor fragilis N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O low molecular weight form EII Mucor fragilis 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O low molecular weight form EII Mucor fragilis 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 tri-N-acetylchitotriose + H2O low molecular weight form EII Mucor fragilis N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer 2 * 70000, low molecular weight form EII Mucor fragilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 45
-
 low molecular weight form EII, stable for 10 min Mucor fragilis
3.2.1.52 57.5
-
 low molecular weight form EII, 50% of activity remains after 10 min Mucor fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4 5 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, low molecular weight form EII Mucor fragilis
3.2.1.52 5.5 6.5 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, low molecular weight form EII Mucor fragilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 4.5 8.5 low molecular weight form EII Mucor fragilis