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Literature summary extracted from
- Purification and properties of the endo-1,4-beta-glucanase from Bacillus subtilis (1987), J. Gen. Microbiol., 133, 2155-2162.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | arabitol | 50 mM, stimulates | Bacillus subtilis |  |
| 3.2.1.4 | dithiothreitol | 5 mM, stimulates | Bacillus subtilis | |
| 3.2.1.4 | erythritol | 50 mM, stimulates | Bacillus subtilis | |
| 3.2.1.4 | Gly | 50 mM, stimulates | Bacillus subtilis | |
| 3.2.1.4 | glycerol | 50 mM, stimulates | Bacillus subtilis | |
| 3.2.1.4 | histamine | 20 mM, stimulates | Bacillus subtilis | |
| 3.2.1.4 | L-His | 50 mM, stimulates | Bacillus subtilis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | Ag+ | - |
Bacillus subtilis | |
| 3.2.1.4 | Cu2+ | - |
Bacillus subtilis | |
| 3.2.1.4 | EDTA | - |
Bacillus subtilis | |
| 3.2.1.4 | Fe2+ | - |
Bacillus subtilis | |
| 3.2.1.4 | Hg2+ | - |
Bacillus subtilis | |
| 3.2.1.4 | Pb2+ | - |
Bacillus subtilis | |
| 3.2.1.4 | Sn2+ | - |
Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.4 | additional information | - |
additional information | - |
Bacillus subtilis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | Co2+ | increases activity by 139.5% | Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 23000 | - |
gel filtration | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | - |
Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | carboxymethylcellulose + H2O | - |
Bacillus subtilis | additional information | glucose + cellobiose | ? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 70 | - |
60 min, stable | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 5.5 | - |
- |
Bacillus subtilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 5.5 | 8 | 50°C, over 95% of the activity is retained | Bacillus subtilis |
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