BRENDA - Enzyme Database

Purification and characterization of Bombyx mori chitinases

Koga, D.; Sasaki, Y.; Uchiumi, Y.; Hirai, N.; Arakane, Y.; Nagamatsu, Y.; Insect Biochem. Mol. Biol. 27, 757-767 (1997) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.14
chitopentaose
-
Bombyx mori
3.2.1.14
CuSO4
isoenzyme 1
Bombyx mori
3.2.1.14
HgCl2
both isoenzymes
Bombyx mori
3.2.1.14
tetraacetylchitotetraose
-
Bombyx mori
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.14
additional information
-
additional information
isoenzyme 1 glycol chitin 0.134 mg/ml, isoenzyme 2 glycol chitin 0.023 mg/ml
Bombyx mori
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.14
65000
-
x * 65000, SDS-PAGE, isoenzyme 1, x * 88000, SDS-PAGE, isoenzyme 2
Bombyx mori
3.2.1.14
88000
-
x * 65000, SDS-PAGE, isoenzyme 1, x * 88000, SDS-PAGE, isoenzyme 2
Bombyx mori
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.14
Bombyx mori
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.14
glycoprotein
65 and 88 kDa isoenzymes
Bombyx mori
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.14
isoenzyme 1, 558fold to homogeneity, isoenzyme 2, 262fold to homogeneity
Bombyx mori
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.14
larva
fifth-instar larvae integument
Bombyx mori
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.14
additional information
-
-
Bombyx mori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.14
chitohexaose + H2O
-
393539
Bombyx mori
chitobiose + chitotriose
and chitotetraose
393539
Bombyx mori
?
3.2.1.14
chitopentaose + H2O
-
393539
Bombyx mori
chitobiose + chitotriose
-
393539
Bombyx mori
?
3.2.1.14
chitotetraose + H2O
-
393539
Bombyx mori
N-acetylglucosamine + chitobiose + chitotriose
chitobiose only
393539
Bombyx mori
?
3.2.1.14
glycol chitin + H2O
-
393539
Bombyx mori
N-acetylglucosamine
-
-
-
?
3.2.1.14
N,N',N''-triacetylchitotriose + H2O
-
393539
Bombyx mori
N-acetylglucosamine + N,N'-diacetylchitobiose
-
393539
Bombyx mori
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.14
?
x * 65000, SDS-PAGE, isoenzyme 1, x * 88000, SDS-PAGE, isoenzyme 2
Bombyx mori
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.14
60
-
both isoenzymes
Bombyx mori
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.14
30
-
stable below 30°C, isoenzyme 1
Bombyx mori
3.2.1.14
40
-
stable below 40°C
Bombyx mori
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.14
0.059
-
glycol chitin
isoenzyme 2
Bombyx mori
3.2.1.14
0.084
-
glycol chitin
isoenzyme 1
Bombyx mori
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.14
5.5
-
N-acetylchitopentaose, isoenzyme 1
Bombyx mori
3.2.1.14
6.5
-
N-acetylchitopentaose, isoenzyme 2
Bombyx mori
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.14
4
10
both isoenzymes
Bombyx mori
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.14
5
11
isoenzyme 1
Bombyx mori
3.2.1.14
6
12
isoenzyme 2
Bombyx mori
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.14
chitopentaose
-
Bombyx mori
3.2.1.14
CuSO4
isoenzyme 1
Bombyx mori
3.2.1.14
HgCl2
both isoenzymes
Bombyx mori
3.2.1.14
tetraacetylchitotetraose
-
Bombyx mori
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.14
additional information
-
additional information
isoenzyme 1 glycol chitin 0.134 mg/ml, isoenzyme 2 glycol chitin 0.023 mg/ml
Bombyx mori
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.14
65000
-
x * 65000, SDS-PAGE, isoenzyme 1, x * 88000, SDS-PAGE, isoenzyme 2
Bombyx mori
3.2.1.14
88000
-
x * 65000, SDS-PAGE, isoenzyme 1, x * 88000, SDS-PAGE, isoenzyme 2
Bombyx mori
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.14
glycoprotein
65 and 88 kDa isoenzymes
Bombyx mori
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.14
isoenzyme 1, 558fold to homogeneity, isoenzyme 2, 262fold to homogeneity
Bombyx mori
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.14
larva
fifth-instar larvae integument
Bombyx mori
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.14
additional information
-
-
Bombyx mori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.14
chitohexaose + H2O
-
393539
Bombyx mori
chitobiose + chitotriose
and chitotetraose
393539
Bombyx mori
?
3.2.1.14
chitopentaose + H2O
-
393539
Bombyx mori
chitobiose + chitotriose
-
393539
Bombyx mori
?
3.2.1.14
chitotetraose + H2O
-
393539
Bombyx mori
N-acetylglucosamine + chitobiose + chitotriose
chitobiose only
393539
Bombyx mori
?
3.2.1.14
glycol chitin + H2O
-
393539
Bombyx mori
N-acetylglucosamine
-
-
-
?
3.2.1.14
N,N',N''-triacetylchitotriose + H2O
-
393539
Bombyx mori
N-acetylglucosamine + N,N'-diacetylchitobiose
-
393539
Bombyx mori
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.14
?
x * 65000, SDS-PAGE, isoenzyme 1, x * 88000, SDS-PAGE, isoenzyme 2
Bombyx mori
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.14
60
-
both isoenzymes
Bombyx mori
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.14
30
-
stable below 30°C, isoenzyme 1
Bombyx mori
3.2.1.14
40
-
stable below 40°C
Bombyx mori
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.14
0.059
-
glycol chitin
isoenzyme 2
Bombyx mori
3.2.1.14
0.084
-
glycol chitin
isoenzyme 1
Bombyx mori
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.14
5.5
-
N-acetylchitopentaose, isoenzyme 1
Bombyx mori
3.2.1.14
6.5
-
N-acetylchitopentaose, isoenzyme 2
Bombyx mori
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.14
4
10
both isoenzymes
Bombyx mori
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.14
5
11
isoenzyme 1
Bombyx mori
3.2.1.14
6
12
isoenzyme 2
Bombyx mori