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Literature summary extracted from

  • Kirsch, C.; Hahlbrock, K.; Kombrink, E.
    Purification and characterization of extracellular, acidic chitinase isoenzymes from elicitor-stimulated parsley cells (1993), Eur. J. Biochem., 213, 419-425.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Petroselinum crispum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 30400
-
 x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE Petroselinum crispum
3.2.1.14 32700
-
 x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE Petroselinum crispum
3.2.1.14 35100
-
 x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE Petroselinum crispum
3.2.1.14 36800
-
 x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE Petroselinum crispum
3.2.1.14 37000
-
 x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE Petroselinum crispum
3.2.1.14 38200
-
 x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE Petroselinum crispum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Petroselinum crispum
-
 parsley, treated with fungal elicitor
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.14 glycoprotein isoenzymes C1, C2, C4, isoenzymes C3 and C4 contain xylose complexes Petroselinum crispum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 all six isoenzymes Petroselinum crispum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 culture filtrate
-
 Petroselinum crispum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 0.0047 0.01 isoenzymes C2 and B, other isoenzymes in between Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O [acetyl-3H]chitin Petroselinum crispum N,N-diacetylchitobiose + N-acetylglucosamine isoenzymes A and B chitotetraose and chitotriose main products, also chitobiose and higher chitooligomers, isoenzymes C1-C4 main product chitobiose, also N-acetylglucosamine and some chitotriose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE Petroselinum crispum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 4
-
 isoenzyme A Petroselinum crispum
3.2.1.14 5
-
 isoenzyme B Petroselinum crispum
3.2.1.14 6
-
 isoenzyme C2 Petroselinum crispum
3.2.1.14 6.5
-
 isoenzymes C1, C3 Petroselinum crispum
3.2.1.14 7
-
 isoenzyme C4 Petroselinum crispum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 9
-
 50% of activity isoenzymes C1-C4 Petroselinum crispum