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Literature summary extracted from

  • Skujins, J.; Pukite, A.; McLaren, A.D.
    Chitinase of Streptomyces sp.: Purification and properties (1970), Enzymologia, 39, 353-370.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.14
-
 Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 AlCl3 deletorious effect at 0.01 M Streptomyces sp.
3.2.1.14 Co2+ inhibiting above 1 mM Streptomyces sp.
3.2.1.14 Mg2+
-
 Streptomyces sp.
3.2.1.14 NaCl
-
 Streptomyces sp.
3.2.1.14 Zn2+ inhibiting above 1 mM Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Ca2+ necessary in small amounts in order to retain enyzmatic activity during purification Streptomyces sp.
3.2.1.14 Co2+ activating below 1 mM Streptomyces sp.
3.2.1.14 Zn2+ activating below 1 mM Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 29000
-
 equilibrium sedimentation Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Streptomyces sp.
-
-
-
3.2.1.14 Streptomyces sp. 2B
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 to homogeneity Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 culture filtrate
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 0.289
-
-
 Streptomyces sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.14 2°C, lycophilized, after purifiaction step 5, stable Streptomyces sp.
3.2.1.14 frozen, prolonged periods, after purification step 4, stable Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Streptomyces sp. N,N-diacetylchitobiose + N-acetylglucosamine N-acetylhexosamine ?
3.2.1.14 chitin + H2O
-
 Streptomyces sp. 2B N,N-diacetylchitobiose + N-acetylglucosamine N-acetylhexosamine ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 65
-
 loss of all activity after 3 h Streptomyces sp.
3.2.1.14 70
-
 loss of all activity after 5 min, 80% of activity remains when dried Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 4.2
-
 Na-phosphate buffer Streptomyces sp.
3.2.1.14 4.8
-
 Na-acetate buffer, sodium phosphate-acetate buffer Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 11
-
 inactivated above pH 11 Streptomyces sp.