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Literature summary extracted from

  • Ellermann, J.; Rospert, S.; Thauer, R.K.; Bokranz, M.; Klein, A.; Voges, M.; Berkessel, A.
    Methyl-coenzyme-M reductase from Methanobacterium thermoautotrophicum (strain Marburg). Purity, activity and novel inhibitors (1989), Eur. J. Biochem., 184, 63-68.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.4.1 ADP in absence of reducing agents Methanothermobacter thermautotrophicus
2.8.4.1 arsenate in absence of reducing agents Methanothermobacter thermautotrophicus
2.8.4.1 ATP
-
 Methanothermobacter thermautotrophicus
2.8.4.1 Cobalamin
-
 Methanothermobacter thermautotrophicus
2.8.4.1 dithiothreitol
-
 Methanothermobacter thermautotrophicus
2.8.4.1 EDTA in absence of reducing agents Methanothermobacter thermautotrophicus
2.8.4.1 additional information activated enzyme immediately loses 97% of activity when assayed in the absence of Ti(III) citrate, dithiothreitol and cobalamin, that shows the requirement of these reductants for maximal activity Methanothermobacter thermautotrophicus
2.8.4.1 phosphate in absence of reducing agents Methanothermobacter thermautotrophicus
2.8.4.1 sulfate in absence of reducing agents Methanothermobacter thermautotrophicus
2.8.4.1 Ti(III) citrate
-
 Methanothermobacter thermautotrophicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.4.1 expression of two ORF gene products in Escherichia coli DS 410, expressed proteins are suggested to be important for enzyme activity, but neither stimulatory nor inhibitory effects of these gene products are determined Methanothermobacter thermautotrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.4.1 2-azidoethanesulfonate competitive, reversible Methanothermobacter thermautotrophicus
2.8.4.1 2-bromoethanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 3-azidopropanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 3-bromopropanesulfonate competitive, reversible, 50% inhibition at 0.00007 mM Methanothermobacter thermautotrophicus
2.8.4.1 3-hydroxypropanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 4-bromobutanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 chloromethanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 ethanol concentrations above 0.005 ml/0.2 ml assay Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.4.1 0.075
-
 coenzyme B
-
 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.1 Ni(2+) nickel-porphinoid-containing protein Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.4.1 additional information
-
 operon contains two additional polypeptides of 15752 and 21210 Da with unknown functions Methanothermobacter thermautotrophicus
2.8.4.1 37000
-
 2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE Methanothermobacter thermautotrophicus
2.8.4.1 48000
-
 2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE Methanothermobacter thermautotrophicus
2.8.4.1 66000
-
 2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE Methanothermobacter thermautotrophicus
2.8.4.1 300000
-
-
 Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothermobacter thermautotrophicus
-
-
-
2.8.4.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.1 membrane filtration, on Mono Q, Superose-6 and Phenyl-Superose column Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.4.1 2.5
-
 purified, addition of Ti(III) citrate, dithiothreitol and cobalamin Methanothermobacter thermautotrophicus
2.8.4.1 3 5 cell extract Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
2.8.4.1 -80°C, under N2 atmosphere, 50 mM PIPES, pH 7.2, 40% v/v glycerol, placed in H2 gas phase, stable for weeks Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 methyl-coenzyme M + coenzyme B
-
 Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl-coenzyme M + coenzyme B
-
 Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.4.1 hexamer alpha2,beta2,gamma2 Methanothermobacter thermautotrophicus
2.8.4.1 hexamer 2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.4.1 62
-
 assay Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.4.1 7
-
 assay at Methanothermobacter thermautotrophicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.4.1 0.00005
-
 3-bromopropanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 0.001
-
 2-azidoethanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 0.004
-
 2-bromoethanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 0.006
-
 4-bromobutanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 0.04
-
 3-azidopropanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 0.25
-
 chloromethanesulfonate
-
 Methanothermobacter thermautotrophicus
2.8.4.1 4
-
 3-hydroxypropanesulfonate
-
 Methanothermobacter thermautotrophicus