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Literature summary extracted from

  • Hoober, K.L.; Joneja, B.; White, H.B.; Thorpe, C.
    A sulfhydryl oxidase from chicken egg white (1996), J. Biol. Chem., 271, 30510-30516.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.2 iodoacetamide
-
Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.2 0.15
-
dithiothreitol
-
Gallus gallus
1.8.3.2 10.9
-
L-Cys
-
Gallus gallus
1.8.3.2 20
-
GSH
-
Gallus gallus
1.8.3.2 54
-
2-mercaptoethanol
-
Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.2 93000
-
2 * 93000, SDS-PAGE Gallus gallus
1.8.3.2 202000
-
gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.2 Gallus gallus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.8.3.2 glycoprotein
-
Gallus gallus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.2
-
Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.3.2 egg egg white Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
1.8.3.2 -20°C, stable for months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.2 2-mercaptoethanol + O2
-
Gallus gallus ? + H2O
-
?
1.8.3.2 dithiothreitol + O2 anaerobically, the ferricenium ion is a facile alternative electron acceptor Gallus gallus ? + H2O production of H2O2 ?
1.8.3.2 GSH + O2 + O2
-
Gallus gallus GSSG + H2O
-
?
1.8.3.2 L-Cys + O2
-
Gallus gallus ? + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.8.3.2 dimer 2 * 93000, SDS-PAGE Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.2 17.2
-
dithiothreitol
-
Gallus gallus
1.8.3.2 20.3
-
2-mercaptoethanol
-
Gallus gallus
1.8.3.2 21.3
-
L-Cys
-
Gallus gallus
1.8.3.2 23.1
-
GSH
-
Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.2 7.5
-
-
Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.3.2 5 11 pH 5.0: about 40% of maximal activity, pH 11: about 70% of maximal activity Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.3.2 FAD
-
Gallus gallus