EC Number | General Stability | Organism |
---|---|---|
1.8.1.2 | low ionic strength such as 0.01 M phosphate buffer destroys NADPH-dependent activities | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.2 | arsenite | - |
Saccharomyces cerevisiae | |
1.8.1.2 | CN- | severe inhibition of NADPH-sulfite,-nitrite,-hydroxylamine reductase, no inhibition of methyl viologen-sulfite reductase, small extent of inhibition of NADPH dependent reduction of cytochrome c, ferricyanide, quinones etc. | Saccharomyces cerevisiae | |
1.8.1.2 | NADP+ | NADPH-dependent activities | Saccharomyces cerevisiae | |
1.8.1.2 | p-chloromercuribenzoate | NADPH-dependent activities | Saccharomyces cerevisiae | |
1.8.1.2 | Sulfide | reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.2 | 0.012 | 0.017 | sulfite | - |
Saccharomyces cerevisiae | |
1.8.1.2 | 0.018 | - |
NADPH | reduction of cytochrome c | Saccharomyces cerevisiae | |
1.8.1.2 | 0.021 | - |
NADPH | reduction of sulfite | Saccharomyces cerevisiae | |
1.8.1.2 | 0.06 | - |
cytochrome c | - |
Saccharomyces cerevisiae | |
1.8.1.2 | 0.8 | 1.5 | nitrite | - |
Saccharomyces cerevisiae | |
1.8.1.2 | 4.5 | 10.5 | hydroxylamine | - |
Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.1.2 | soluble | - |
Saccharomyces cerevisiae | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.2 | 350000 | - |
calculation from FAD and FMN content, sedimentation coefficient | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.2 | sulfite + NADPH + H+ | Saccharomyces cerevisiae | - |
sulfide + NADP+ + H2O | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.8.1.2 | Acetone | inactivation | Saccharomyces cerevisiae |
1.8.1.2 | Ethanol | inactivation | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.2 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.2 | - |
Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
1.8.1.2 | -20°C, 0.3 M phosphate buffer pH 7.3, 1 year stable stable | Saccharomyces cerevisiae |
1.8.1.2 | 4°C, 0.3 M phosphate buffer pH 7.3, 1 week stable | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.2 | hydroxylamine + NADPH | - |
Saccharomyces cerevisiae | ammonia + NADP+ | - |
? | |
1.8.1.2 | additional information | overview on assay methods | Saccharomyces cerevisiae | ? | - |
? | |
1.8.1.2 | additional information | reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 | Saccharomyces cerevisiae | ? | - |
? | |
1.8.1.2 | NADP+ + ferrocytochrome c | - |
Saccharomyces cerevisiae | NADPH + H+ + ferricytochrome c | - |
r | |
1.8.1.2 | nitrite + NADPH | - |
Saccharomyces cerevisiae | ammonia + NADP+ | - |
? | |
1.8.1.2 | sulfite + NADPH | - |
Saccharomyces cerevisiae | sulfide + NADP+ + H2O | - |
? | |
1.8.1.2 | sulfite + NADPH + H+ | - |
Saccharomyces cerevisiae | sulfide + NADP+ + H2O | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.1.2 | 7 | 7.5 | NADPH-sulfite reductase | Saccharomyces cerevisiae |
1.8.1.2 | 7.5 | 7.9 | NADPH-cytochrome c reductase | Saccharomyces cerevisiae |
1.8.1.2 | 7.7 | 8.5 | methyl viologen-sulfite reductase | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.2 | flavin | treatment with ammonium sulfate causes dissociation of FMN but retetion of FAD and heme | Saccharomyces cerevisiae |