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Literature summary extracted from

  • Vega, J.M.; Guerrero, M.G.; Leadbetter, E.; Losada, M.
    Reduced nicotinamide-adenine dinucleotide-nitrite reductase from Azotobacter chroococcum (1973), Biochem. J., 133, 701-708.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.4 FMN no effect Azotobacter chroococcum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.4 Carbamoyl phosphate
-
 Azotobacter chroococcum
1.7.1.4 CN- 0.01 mM, 40% inhibition, 0.1 mM, 93% inhibition Azotobacter chroococcum
1.7.1.4 CO competitive vs. nitrite Azotobacter chroococcum
1.7.1.4 Cyanate
-
 Azotobacter chroococcum
1.7.1.4 NAD(P)H inactivation after preincubation, presence of nitrite protects from inactivation Azotobacter chroococcum
1.7.1.4 NaN3
-
 Azotobacter chroococcum
1.7.1.4 p-hydroxymercuribenzoate 0.01 mM, 42% inhibition, 0.1 mM, complete inhibition Azotobacter chroococcum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.4 0.0055
-
 NO2-
-
 Azotobacter chroococcum
1.7.1.4 0.015
-
 NADH
-
 Azotobacter chroococcum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.4 67000
-
 sucrose density gradient centrifugation Azotobacter chroococcum

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.4 Azotobacter chroococcum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.4 NAD(P)H + nitrite + H+ NADH is preferred, S2O42- can act as electron donor, methyl viologen as electron carrier Azotobacter chroococcum NAD(P)+ + NH3 + H2O
-
 ?
1.7.1.4 nitrite + NADH + H+
-
 Azotobacter chroococcum NH3 + NAD+ + H2O
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.4 FAD stimulation Azotobacter chroococcum
1.7.1.4 additional information FMN has no effect Azotobacter chroococcum
1.7.1.4 NADH
-
 Azotobacter chroococcum
1.7.1.4 NADPH
-
 Azotobacter chroococcum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.1.4 0.000032
-
 CO
-
 Azotobacter chroococcum