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Literature summary extracted from
- Thiol modification and site directed mutagenesis of the flavin domain of spinach NADH:nitrate reductase (1996), Arch. Biochem. Biophys., 331, 117-126.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.7.1.1 | C17S | visible and CD spectra are very similar to that of the wild-type domain, thermal stability is slightly decreased compared to the wild-type domain | Spinacia oleracea |
| 1.7.1.1 | C240A | decrease of diaphorase activity | Spinacia oleracea |
| 1.7.1.1 | C240G | decrease of diaphorase activity | Spinacia oleracea |
| 1.7.1.1 | C240S | decrease of diaphorase activity, thermal stability is slightly decreased compared to the wild-type domain, the oxidation reduction midpoint potential for the FAD/FADH2 couple is -219 mV compared to -268 mV in the wild-type domain | Spinacia oleracea |
| 1.7.1.1 | C54S | visible and CD spectra are very similar to that of the wild-type domain, thermal stability is slightly decreased compared to the wild-type domain,the oxidation reduction midpoint potential for the FAD/FADH2 couple is -197 mV compared to -268 mV in the wild-type domain | Spinacia oleracea |
| 1.7.1.1 | C62S | visible and CD spectra are very similar to that of the wild-type domain, thermal stability is decreased compared to the wild-type domain, the oxidation reduction midpoint potential for the FAD/FADH2 couple is -226 mV compared to -268 mV in the wild-type domain | Spinacia oleracea |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.1.1 | 2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid | - |
Chlorella sp. |  |
| 1.7.1.1 | 2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid | - |
Spinacia oleracea | |
| 1.7.1.1 | methyl methanethiosulfonate | - |
Chlorella sp. | |
| 1.7.1.1 | methyl methanethiosulfonate | inactivation is concentration independent | Spinacia oleracea | |
| 1.7.1.1 | NAD+ | - |
Spinacia oleracea | |
| 1.7.1.1 | NEM | - |
Chlorella sp. | |
| 1.7.1.1 | NEM | - |
Spinacia oleracea | |
| 1.7.1.1 | PCMB | - |
Chlorella sp. | |
| 1.7.1.1 | PCMB | inactivation is concentration independent | Spinacia oleracea | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.7.1.1 | 0.004 | - |
NADH | recombinant flavin domain of nitrate reductase C240A mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.011 | - |
NADH | recombinant flavin domain of nitrate reductase C240G mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.012 | - |
NADH | wild-type recombinant flavin domain of nitrate reductase | Spinacia oleracea | |
| 1.7.1.1 | 0.014 | - |
NADH | recombinant flavin domain of nitrate reductase C240S mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.015 | - |
NADH | recombinant flavin domain of nitrate reductase C17S mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.015 | - |
NADH | recombinant flavin domain of nitrate reductase C54S mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.016 | - |
NADH | recombinant flavin domain of nitrate reductase C62S mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.024 | - |
NADH | recombinant flavin domain of nitrate reductase C240S mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.027 | - |
ferricyanide | wild-type recombinant flavin domain of nitrate reductase | Spinacia oleracea | |
| 1.7.1.1 | 0.036 | - |
ferricyanide | recombinant flavin domain of nitrate reductase C54S mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.045 | - |
ferricyanide | recombinant flavin domain of nitrate reductase C17S mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.059 | - |
NADH | recombinant flavin domain of nitrate reductase C62S mutant | Spinacia oleracea | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.7.1.1 | Chlorella sp. | - |
- |
- |
| 1.7.1.1 | Spinacia oleracea | - |
recombinant flavin domain | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.1.1 | 2 ferricyanide + NADH | - |
Spinacia oleracea | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| 1.7.1.1 | 2 ferricyanide + NADH | - |
Chlorella sp. | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| 1.7.1.1 | nitrate + NADH | - |
Spinacia oleracea | nitrite + NAD+ + H2O | - |
? | |
| 1.7.1.1 | nitrate + NADH | - |
Chlorella sp. | nitrite + NAD+ + H2O | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.1.1 | NADH | - |
Spinacia oleracea | |
| 1.7.1.1 | NADH | - |
Chlorella sp. | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.7.1.1 | 0.5 | - |
NAD+ | recombinant flavin domain of nitrate reductase C240G mutant | Spinacia oleracea | |
| 1.7.1.1 | 0.8 | - |
NAD+ | recombinant flavin domain of nitrate reductase C240A mutant | Spinacia oleracea | |
| 1.7.1.1 | 1.2 | - |
NAD+ | recombinant flavin domain of nitrate reductase C240S mutant | Spinacia oleracea | |
| 1.7.1.1 | 1.5 | - |
NAD+ | recombinant flavin domain of nitrate reductase C54S mutant | Spinacia oleracea | |
| 1.7.1.1 | 1.9 | - |
NAD+ | recombinant flavin domain of nitrate reductase wild-type and C17S mutant | Spinacia oleracea | |
| 1.7.1.1 | 2 | - |
NAD+ | recombinant flavin domain of nitrate reductase C62S mutant | Spinacia oleracea | |
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