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Literature summary extracted from

  • Murthy, S.S.; Zilinskas, B.A.
    Molecular cloning and characterization of a cDNA encoding pea monodehydroascorbate reductase (1994), J. Biol. Chem., 269, 31129-31133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.4 expression in Escherichia coli fused to maltose-binding protein Pisum sativum

General Stability

EC Number General Stability Organism
1.6.5.4 4°C, maltose-binding protein-monodehydroascorbate reductase fusion protein, in presence of 2-mercaptoethanol, 1 month, stable Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.4 0.0053
-
 NADH maltose-binding protein-monodehydroascorbate reductase fusion protein Pisum sativum
1.6.5.4 0.0057
-
 monodehydroascorbate maltose-binding protein-monodehydroascorbate reductase fusion protein Pisum sativum
1.6.5.4 0.0215
-
 NADPH maltose-binding protein-monodehydroascorbate reductase fusion protein Pisum sativum
1.6.5.4 0.052
-
 oxidized 2,6-dichlorophenolindophenol maltose-binding protein-monodehydroascorbate reductase fusion protein Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.4 47000
-
 x * 47000, SDS-PAGE Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.4 NADH + monodehydroascorbate Pisum sativum key enzyme in maintaining reduced pools of ascorbate NAD+ + ascorbate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Pisum sativum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 ferricyanide + NADPH + H+
-
 Pisum sativum ferrocyanide + NADP+
-
 r
1.6.5.4 NADH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Pisum sativum NAD+ + reduced 2,6-dichlorophenolindophenol
-
 r
1.6.5.4 NADH + monodehydroascorbate
-
 Pisum sativum NAD+ + ascorbate
-
 ?
1.6.5.4 NADH + monodehydroascorbate key enzyme in maintaining reduced pools of ascorbate Pisum sativum NAD+ + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.4 ? x * 47000, SDS-PAGE Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 FAD flavin enzyme Pisum sativum
1.6.5.4 NADH
-
 Pisum sativum