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Literature summary extracted from
- A new type-II NADH dehydrogenase from the archaeon Acidianus ambivalens: characterization and in vitro reconstitution of the respiratory chain (2001), J. Bioenerg. Biomembr., 33, 1-8.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.1.2 | 0.006 | - |
NADH | - |
Acidianus ambivalens |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | Iron | contains no Fe-S clusters | Acidianus ambivalens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 47000 | - |
1 * 47000, SDS-PAGE | Acidianus ambivalens |
| 7.1.1.2 | 60000 | - |
gel filtration | Acidianus ambivalens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.2 | NADH + ubiquinone | Acidianus ambivalens | enzyme is involved in respiratory chain | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.2 | Acidianus ambivalens | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.2 | 2 ferricyanide + NADH | - |
Acidianus ambivalens | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| 7.1.1.2 | NADH + caldariellaquinone | - |
Acidianus ambivalens | NAD+ + caldariellaquinol | - |
? | |
| 7.1.1.2 | NADH + ubiquinone | - |
Acidianus ambivalens | NAD+ + ubiquinol | - |
? | |
| 7.1.1.2 | NADH + ubiquinone | enzyme is involved in respiratory chain | Acidianus ambivalens | ? | - |
? | |
| 7.1.1.2 | NADH + ubiquinone-1 | - |
Acidianus ambivalens | NAD+ + ubiquinol-1 | - |
? | |
| 7.1.1.2 | NADH + ubiquinone-2 | - |
Acidianus ambivalens | NAD+ + reduced ubiquinol-2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | monomer | 1 * 47000, SDS-PAGE | Acidianus ambivalens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | NADH:caldariella quinone oxidoreductase | - |
Acidianus ambivalens |
| 7.1.1.2 | NDH-II | - |
Acidianus ambivalens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 35 | 80 | activity increases 4fold from 35°C to 80°C, the maximal possible operating temperature | Acidianus ambivalens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | NADH | - |
Acidianus ambivalens | |
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Release 2023.1 (January 2023)
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