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Literature summary extracted from
- Properties of a cysteine-free proton-pumping nicotinamide nucleotide transhydrogenase (1997), Biochem. J., 324, 681-687.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.1.1.1 | C292T/C339T/C395S/C397T/C435S | cysteine of the alpha subunits replaced, similar activity as wild-type | Escherichia coli |
| 7.1.1.1 | C292T/C339T/C395S/C397T/C435S/C147S/C260S | all 7 cysteines of the enzyme, 5 localized in the alpha subunit and 2 in the beta subunit, are replaced, the cysteine-free mutant shows about 5fold more activity in the reduction of acetylpyridine adenine dinucleotide by NADH than wild-type, the cyclic reduction of acetylpyridine adenine dinucleotide by NADH via NADPH is 2-2.5fold more activ | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.1 | N-ethylmaleimide | cysteine-free mutant enzyme is not inhibited | Escherichia coli |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.1.1 | 0.0017 | - |
NADH | wild-type enzyme | Escherichia coli | |
| 7.1.1.1 | 0.002 | - |
NADH | cysteine-free enzyme | Escherichia coli | |
| 7.1.1.1 | 0.0051 | - |
NADPH | cysteine-free enzyme | Escherichia coli | |
| 7.1.1.1 | 0.008 | - |
thio-NADP+ | wild-type enzyme | Escherichia coli | |
| 7.1.1.1 | 0.015 | - |
NADPH | wild-type enzyme | Escherichia coli | |
| 7.1.1.1 | 0.018 | - |
thio-NADP+ | cysteine-free enzyme | Escherichia coli | |
| 7.1.1.1 | 0.02 | - |
oxidized acetylpyridine adenine dinucleotide | cysteine-free enzyme | Escherichia coli | |
| 7.1.1.1 | 0.026 | - |
oxidized acetylpyridine adenine dinucleotide | wild-type enzyme | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.1 | Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.1 | 0.2 | - |
reduction of NADP+ by NADH driven by electron transport, cysteine-free enzyme reconstituted in membrane vesicles | Escherichia coli |
| 7.1.1.1 | 0.42 | - |
reduction of NADP+ by NADH driven by electron transport, wild-type enzyme reconstituted in membrane vesicles | Escherichia coli |
| 7.1.1.1 | 1.9 | - |
reduction of acetylpyridine adenine dinucleotide by NADPH, cysteine-free enzyme reconstituted in membrane vesicles | Escherichia coli |
| 7.1.1.1 | 3 | - |
reduction of acetylpyridine adenine dinucleotide by NADPH, wild-type enzyme reconstituted in membrane vesicles | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.1 | NAD+ | - |
Escherichia coli | |
| 7.1.1.1 | NADH | - |
Escherichia coli | |
| 7.1.1.1 | NADP+ | - |
Escherichia coli | |
| 7.1.1.1 | NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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