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Literature summary extracted from

  • Noda, C.; Ichihara, A.
    Purification and properties of L-lysine-alpha-ketoglutarate reductase from rat liver mitochondria (1978), Biochim. Biophys. Acta, 525, 307-313.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.8 L-ornithine complete inhibition of saccharopine formation, competitive with lysine Rattus norvegicus
1.5.1.8 leucine complete inhibition of saccharopine formation, competitive with lysine Rattus norvegicus
1.5.1.8 tryptophan complete inhibition of saccharopine formation Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.8 0.078
-
 NADPH
-
 Rattus norvegicus
1.5.1.8 1.4
-
 2-oxoglutarate
-
 Rattus norvegicus
1.5.1.8 2.2
-
 L-lysine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.8 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.8 52000
-
 4 * 52000, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9, SDS-PAGE Rattus norvegicus
1.5.1.8 230000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.8 Rattus norvegicus
-
 male Wistar strain
-
1.5.1.8 Rattus norvegicus male Wistar
-
 male Wistar strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.8 300fold purification, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.8 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.8 28.3
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.5.1.8 0°C, 10 mM Tris-HCl buffer, pH 7.6, 5 mM 2-mercaptoethanol, 0.1 mM EDTA, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O lysine formation is only 3-5% of the saccharopine formation r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH equilibrium favors sacharopine formation, lysine formation is only 3-5% of the saccharopine formation Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O lysine formation is only 3-5% of the saccharopine formation r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH equilibrium favors sacharopine formation, lysine formation is only 3-5% of the saccharopine formation Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O lysine formation is only 3-5% of the saccharopine formation r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Rattus norvegicus N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Rattus norvegicus male Wistar N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O lysine formation is only 3-5% of the saccharopine formation r
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Rattus norvegicus male Wistar N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
1.5.1.8 additional information not as electron acceptor: NADH Rattus norvegicus ?
-
 ?
1.5.1.8 additional information lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 are separate enzymes Rattus norvegicus ?
-
 ?
1.5.1.8 additional information not as substrates: ornithine, glutamine, oxaloacetate, pyruvate Rattus norvegicus ?
-
 ?
1.5.1.8 additional information not as electron acceptor: NADH Rattus norvegicus male Wistar ?
-
 ?
1.5.1.8 additional information lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 are separate enzymes Rattus norvegicus male Wistar ?
-
 ?
1.5.1.8 additional information not as substrates: ornithine, glutamine, oxaloacetate, pyruvate Rattus norvegicus male Wistar ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.8 homotetramer 4 * 52000, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.8 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.8 8.4
-
 saccharopine formation Rattus norvegicus
1.5.1.8 9
-
 L-lysine formation Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.8 NADP+
-
 Rattus norvegicus
1.5.1.8 NADPH specific for NADPH Rattus norvegicus