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Literature summary extracted from

  • Cossins, E.A.; Wong, K.F.; Roos, A.J.
    Plant N5,N10-methylenetetrahydrofolate dehydrogenase: partial purification and some general properties of the enzyme from germinating pea seedlings (1970), Phytochemistry, 9, 1463-1471.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.5.1.5 relatively instable after partial purification Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.5 2,2'-dipyridyl
-
 Pisum sativum
1.5.1.5 iodoacetate
-
 Pisum sativum
1.5.1.5 o-phenanthroline
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.5 0.0004
-
 NADP+
-
 Pisum sativum
1.5.1.5 0.21
-
 DL-tetrahydrofolic acid
-
 Pisum sativum
1.5.1.5 0.79
-
 formaldehyde
-
 Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Pisum sativum
-
 L. cv. Homesteader
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.5 partial Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.5 cotyledon
-
 Pisum sativum
-
1.5.1.5 root
-
 Pisum sativum
-
1.5.1.5 shoot
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.5 additional information
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
1.5.1.5 2-4°C, 10% loss of activity in 4 days Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 DL-tetrahydrofolic acid + NADP+
-
 Pisum sativum ?
-
 ?
1.5.1.5 formaldehyde + NADP+
-
 Pisum sativum ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.5 6.7
-
-
 Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.5 6 7
-
 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NAD+ 23% of the activity with NADP+ Pisum sativum
1.5.1.5 NADP+
-
 Pisum sativum