BRENDA - Enzyme Database

Deoxyribonucleotide biosynthesis in green algae: characterization of thymidylate synthase-dihydrofolate reductase in Scenedesmus obliquus

Bachmann, B.; Follmann, H.; Arch. Biochem. Biophys. 256, 244-252 (1987)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.1.1.45
-80C, storage in 30% glycerol stabilizes enzyme
Tetradesmus obliquus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.5.1.3
folate
-
Tetradesmus obliquus
1.5.1.3
methotrexate
-
Tetradesmus obliquus
1.5.1.3
additional information
thymidylate synthase activity inhibitors
Tetradesmus obliquus
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Tetradesmus obliquus
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Daucus carota
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Saccharomyces cerevisiae
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Leishmania major
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Mus musculus
2.1.1.45
Arabinouridylate
-
Tetradesmus obliquus
2.1.1.45
Hydroxyurea
supresses thymidilate synthase in growing algae, but no effect on purified enzyme
Tetradesmus obliquus
2.1.1.45
methotrexate
-
Tetradesmus obliquus
2.1.1.45
methotrexate
-
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.5.1.3
50000
-
2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE
Tetradesmus obliquus
1.5.1.3
100000
-
thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, sucrose density gradient centrifugation
Tetradesmus obliquus
2.1.1.45
50000
-
2 * 50000, SDS-PAGE
Tetradesmus obliquus
2.1.1.45
56000
-
2 * 56000
Leishmania major
2.1.1.45
100000
-
sedimentation in sucrose density gradients
Tetradesmus obliquus
2.1.1.45
110000
-
-
Leishmania major
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Chlamydomonas reinhardtii
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Tetradesmus obliquus
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Mus musculus
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Saccharomyces cerevisiae
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Bos taurus
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Nicotiana tabacum
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Daucus carota
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Leishmania major
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Abies sp.
-
dihydrofolate + dTMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.5.1.3
Tetradesmus obliquus
-
green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other
-
2.1.1.45
Abies sp.
-
pine
-
2.1.1.45
Bos taurus
-
calf
-
2.1.1.45
Chlamydomonas reinhardtii
-
-
-
2.1.1.45
Daucus carota
-
carrot, wild and culture form
-
2.1.1.45
Leishmania major
-
-
-
2.1.1.45
Mus musculus
-
-
-
2.1.1.45
Nicotiana tabacum
-
tobacco
-
2.1.1.45
Saccharomyces cerevisiae
-
bakers' yeast
-
2.1.1.45
Tetradesmus obliquus
-
unicellular green algae
-
Purification (Commentary)
EC Number
Commentary
Organism
1.5.1.3
green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other
Tetradesmus obliquus
2.1.1.45
-
Tetradesmus obliquus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.45
thymus
-
Bos taurus
-
2.1.1.45
thymus
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.5.1.3
15.8
-
-
Tetradesmus obliquus
2.1.1.45
0.0257
-
-
Tetradesmus obliquus
Storage Stability
EC Number
Storage Stability
Organism
1.5.1.3
5C, enzyme after affinity chromatography, half-life 2 days
Tetradesmus obliquus
2.1.1.45
-80C, storage in 30% glycerol stabilizes enzyme
Tetradesmus obliquus
2.1.1.45
5C half-life in buffered solution only 2 days
Tetradesmus obliquus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.3
7,8-dihydrofolate + NADPH
-
392255
Tetradesmus obliquus
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Chlamydomonas reinhardtii
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Chlamydomonas reinhardtii
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Tetradesmus obliquus
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Tetradesmus obliquus
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Mus musculus
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Mus musculus
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Saccharomyces cerevisiae
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Saccharomyces cerevisiae
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Bos taurus
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Bos taurus
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Nicotiana tabacum
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Nicotiana tabacum
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Daucus carota
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Daucus carota
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Leishmania major
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Leishmania major
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Abies sp.
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Abies sp.
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
additional information
bifunctional enzyme thymidilate synthase-dihydrofolate reductase, not possible to separate the 2 activities
392255
Tetradesmus obliquus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.5.1.3
dimer
2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE
Tetradesmus obliquus
2.1.1.45
dimer
2 * 56000
Leishmania major
2.1.1.45
dimer
2 * 50000, SDS-PAGE
Tetradesmus obliquus
2.1.1.45
dimer
-
Bos taurus
2.1.1.45
dimer
-
Saccharomyces cerevisiae
2.1.1.45
dimer
-
Mus musculus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.5.1.3
25
30
-
Tetradesmus obliquus
1.5.1.3
25
-
assay at
Tetradesmus obliquus
2.1.1.45
25
30
-
Tetradesmus obliquus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.3
7.5
-
thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other
Tetradesmus obliquus
2.1.1.45
7.5
-
-
Tetradesmus obliquus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.5.1.3
6
9
activity negligible below and above
Tetradesmus obliquus
2.1.1.45
6
9
activity is negligible below pH 6.0 and above pH 9.0
Tetradesmus obliquus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.3
NADPH
-
Tetradesmus obliquus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.3
NADPH
-
Tetradesmus obliquus
General Stability (protein specific)
EC Number
General Stability
Organism
2.1.1.45
-80C, storage in 30% glycerol stabilizes enzyme
Tetradesmus obliquus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.5.1.3
folate
-
Tetradesmus obliquus
1.5.1.3
methotrexate
-
Tetradesmus obliquus
1.5.1.3
additional information
thymidylate synthase activity inhibitors
Tetradesmus obliquus
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Tetradesmus obliquus
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Daucus carota
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Saccharomyces cerevisiae
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Leishmania major
2.1.1.45
5-fluoro-2'-deoxyuridylic acid
-
Mus musculus
2.1.1.45
Arabinouridylate
-
Tetradesmus obliquus
2.1.1.45
Hydroxyurea
supresses thymidilate synthase in growing algae, but no effect on purified enzyme
Tetradesmus obliquus
2.1.1.45
methotrexate
-
Tetradesmus obliquus
2.1.1.45
methotrexate
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.5.1.3
50000
-
2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE
Tetradesmus obliquus
1.5.1.3
100000
-
thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, sucrose density gradient centrifugation
Tetradesmus obliquus
2.1.1.45
50000
-
2 * 50000, SDS-PAGE
Tetradesmus obliquus
2.1.1.45
56000
-
2 * 56000
Leishmania major
2.1.1.45
100000
-
sedimentation in sucrose density gradients
Tetradesmus obliquus
2.1.1.45
110000
-
-
Leishmania major
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Chlamydomonas reinhardtii
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Tetradesmus obliquus
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Mus musculus
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Saccharomyces cerevisiae
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Bos taurus
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Nicotiana tabacum
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Daucus carota
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Leishmania major
-
dihydrofolate + dTMP
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
Abies sp.
-
dihydrofolate + dTMP
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.1.3
green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other
Tetradesmus obliquus
2.1.1.45
-
Tetradesmus obliquus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.45
thymus
-
Bos taurus
-
2.1.1.45
thymus
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.5.1.3
15.8
-
-
Tetradesmus obliquus
2.1.1.45
0.0257
-
-
Tetradesmus obliquus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.5.1.3
5C, enzyme after affinity chromatography, half-life 2 days
Tetradesmus obliquus
2.1.1.45
-80C, storage in 30% glycerol stabilizes enzyme
Tetradesmus obliquus
2.1.1.45
5C half-life in buffered solution only 2 days
Tetradesmus obliquus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.3
7,8-dihydrofolate + NADPH
-
392255
Tetradesmus obliquus
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Chlamydomonas reinhardtii
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Chlamydomonas reinhardtii
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Tetradesmus obliquus
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Tetradesmus obliquus
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Mus musculus
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Mus musculus
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Saccharomyces cerevisiae
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Saccharomyces cerevisiae
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Bos taurus
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Bos taurus
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Nicotiana tabacum
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Nicotiana tabacum
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Daucus carota
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Daucus carota
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Leishmania major
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Leishmania major
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Abies sp.
dihydrofolate + dTMP
-
-
-
?
2.1.1.45
5,10-methylenetetrahydrofolate + dUMP
-
392255
Abies sp.
dihydrofolate + dTMP
-
-
-
r
2.1.1.45
additional information
bifunctional enzyme thymidilate synthase-dihydrofolate reductase, not possible to separate the 2 activities
392255
Tetradesmus obliquus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.5.1.3
dimer
2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE
Tetradesmus obliquus
2.1.1.45
dimer
2 * 56000
Leishmania major
2.1.1.45
dimer
2 * 50000, SDS-PAGE
Tetradesmus obliquus
2.1.1.45
dimer
-
Bos taurus
2.1.1.45
dimer
-
Saccharomyces cerevisiae
2.1.1.45
dimer
-
Mus musculus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.5.1.3
25
30
-
Tetradesmus obliquus
1.5.1.3
25
-
assay at
Tetradesmus obliquus
2.1.1.45
25
30
-
Tetradesmus obliquus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.3
7.5
-
thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other
Tetradesmus obliquus
2.1.1.45
7.5
-
-
Tetradesmus obliquus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.5.1.3
6
9
activity negligible below and above
Tetradesmus obliquus
2.1.1.45
6
9
activity is negligible below pH 6.0 and above pH 9.0
Tetradesmus obliquus