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Literature summary extracted from

  • Dann, J.G.; Ostler, G.; Bjur, R.A.; King, R.W.; Scudder, P.; Turner, P.C.; Roberts, G.C.K.; Burgen, A.S.V.; Harding, N.G.L.
    Large-scale purification and characterization of dihydrofolate reductase from a methotrexate-resistant strain of Lactobacillus casei (1976), Biochem. J., 157, 559-571.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.5.1.3 frozen solutions are not stable for long periods Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 additional information
-
 Lacticaseibacillus casei
1.5.1.3 NADP+
-
 Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Lacticaseibacillus casei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.3 Na+ activates Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 17900
-
 gel filtration Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Lacticaseibacillus casei
-
 methotrexate-resistant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 additional information
-
-
 Lacticaseibacillus casei

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 -20°C, freeze-dried, several months Lacticaseibacillus casei
1.5.1.3 4°C, a few days Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Lacticaseibacillus casei 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH
-
 Lacticaseibacillus casei 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 0.02
-
 folate
-
 Lacticaseibacillus casei
1.5.1.3 4
-
 7,8-dihydrofolate + NADPH Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 7.3
-
-
 Lacticaseibacillus casei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.3 5.9 8.5 half-maximal activities at pH 5.9 and pH 8.5 Lacticaseibacillus casei

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADH
-
 Lacticaseibacillus casei
1.5.1.3 NADPH
-
 Lacticaseibacillus casei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Lacticaseibacillus casei
1.5.1.3 0.0018
-
 NADP+
-
 Lacticaseibacillus casei