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Literature summary extracted from

  • Shiono, T.; Kador, P.F.; Kinoshita, J.J.
    Purification and characterization of rat lens pyrroline-5-carboxylate reductase (1986), Biochim. Biophys. Acta, 881, 72-78.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 ADP
-
 Rattus norvegicus
1.5.1.2 ATP 1 mM Rattus norvegicus
1.5.1.2 Cd2+
-
 Rattus norvegicus
1.5.1.2 Cu2+
-
 Rattus norvegicus
1.5.1.2 Hg2+
-
 Rattus norvegicus
1.5.1.2 NADP+ 1 mM Rattus norvegicus
1.5.1.2 p-chloromercuribenzoate
-
 Rattus norvegicus
1.5.1.2 proline 5 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.006
-
 NADPH
-
 Rattus norvegicus
1.5.1.2 0.022
-
 1-pyrroline-5-carboxylate cosubstrate NADPH Rattus norvegicus
1.5.1.2 0.051
-
 DL-pyrroline-5-carboxylate cosubstrate NADPH Rattus norvegicus
1.5.1.2 0.21
-
 1-pyrroline-5-carboxylate cosubstrate NADH Rattus norvegicus
1.5.1.2 0.37
-
 NADH
-
 Rattus norvegicus
1.5.1.2 0.62
-
 DL-pyrroline-5-carboxylate cosubstrate NADH Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 30000
-
 SDS-PAGE Rattus norvegicus
1.5.1.2 240000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.2 lens
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 720
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Rattus norvegicus L-proline + NAD+
-
 r
1.5.1.2 L-Pro + NAD+
-
 Rattus norvegicus 1-pyrroline-5-carboxylate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.2 octamer 8 * 30000, SDS-PAGE Rattus norvegicus
1.5.1.2 octamer 8 * 30000 SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.2 22
-
 96 h stable Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 6.5 7.1
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADPH preferentially Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.2 0.0037
-
 NADP+ against NADH Rattus norvegicus
1.5.1.2 0.12
-
 ATP against DL-pyrroline-5-carboxylate Rattus norvegicus
1.5.1.2 0.2
-
 ATP against NADH Rattus norvegicus
1.5.1.2 0.32
-
 ATP against NADPH Rattus norvegicus
1.5.1.2 0.38
-
 NADP+ against NADPH Rattus norvegicus