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Literature summary extracted from

  • Klamann, E.; Lingens, F.
    Degradation of (-)-ephedrine by Pseudomonas putida detection of (-)-ephedrine:NAD+-oxidoreductase from Arthrobacter globiformis (1980), Z. Naturforsch. C, 35, 80-87.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.18 enzyme is sensitive to inactivation by dilution Arthrobacter globiformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.18 (-)-ephedrine substrate inhibition at higher concentrations Arthrobacter globiformis
1.5.1.18 additional information EDTA causes no remarkable loss of activity Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.18 0.02
-
 (-)-ephedrine
-
 Arthrobacter globiformis
1.5.1.18 0.11
-
 NAD+
-
 Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.18 (-)-ephedrine + NAD+ Arthrobacter globiformis first step in the catabolism of (-)-ephedrine (R)-2-methylimino-1-phenylpropan-1-ol + NADH
-
 ?
1.5.1.18 (-)-ephedrine + NAD+ Arthrobacter globiformis plays role in the metabolism of quite a number of different aromatic and aliphatic amino alcohols (R)-2-methylimino-1-phenylpropan-1-ol + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.18 Arthrobacter globiformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.18 ammonium sulfate precipitation, 9.1fold enrichment Arthrobacter globiformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.18 additional information
-
 additional information Arthrobacter globiformis
1.5.1.18 0.61
-
-
 Arthrobacter globiformis

Storage Stability

EC Number Storage Stability Organism
1.5.1.18 -20°C, crude extract, 6 months, no appreciable loss of activity Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.18 (+)-norephedrine + NAD+ higher rate than (-)-ephedrine Arthrobacter globiformis 2-imino-1-hydroxy-1-phenylpropane + NADH
-
 ?
1.5.1.18 (+)-norephedrine + NAD+ norephedrine is identical with threo-2-amino-1-hydroxy-1-phenylpropane Arthrobacter globiformis 2-imino-1-hydroxy-1-phenylpropane + NADH
-
 ?
1.5.1.18 (+)-pseudoephedrine + NAD+ higher rate than (-)-ephedrine Arthrobacter globiformis 2-methylimino-1-phenylpropan-1-ol + NADH
-
 ?
1.5.1.18 (+)-pseudoephedrine + NAD+ pseudoephedrine is identical with DL-threo-2-(methylamino)-1-phenylpropan-1-ol Arthrobacter globiformis 2-methylimino-1-phenylpropan-1-ol + NADH
-
 ?
1.5.1.18 (-)-ephedrine + NAD+ (-)-ephedrine is identical with 2-methylamino-1-phenyl-1-propanal Arthrobacter globiformis (R)-2-methylimino-1-phenylpropan-1-ol + NADH phenylacetylcarbinol is identical with 1-hydroxy-1-phenylpropan-2-one ?
1.5.1.18 (-)-ephedrine + NAD+ (-)-ephedrine is identical with 2-methylamino-1-phenyl-1-propanal Arthrobacter globiformis (R)-2-methylimino-1-phenylpropan-1-ol + NADH three products: NADH, methylamine and phenylacetylcarbinol or its tautomeric form methylbenzoylcarbinol, ratio of NADH to methylamine is 1:1.1, enzyme attacks (-)-ephedrine at CH-NHCH3, yielding 2-methylimino-1-phenylpropanol, which is immediately hydrolyzed forming methylamine and phenylacetylcarbinol ?
1.5.1.18 (-)-ephedrine + NAD+ first step in the catabolism of (-)-ephedrine Arthrobacter globiformis (R)-2-methylimino-1-phenylpropan-1-ol + NADH
-
 ?
1.5.1.18 (-)-ephedrine + NAD+ plays role in the metabolism of quite a number of different aromatic and aliphatic amino alcohols Arthrobacter globiformis (R)-2-methylimino-1-phenylpropan-1-ol + NADH
-
 ?
1.5.1.18 (-)-sympatol + NAD+ higher rate than (-)-ephedrine Arthrobacter globiformis 4-[(1R,2E)-1-hydroxy-2-(methylimino)ethyl]phenol + NADH
-
 ?
1.5.1.18 (-)-sympatol + NAD+ sympatol is identical with p-hydroxy-alpha-[(methylamino)methyl]benzyl tartrate Arthrobacter globiformis 4-[(1R,2E)-1-hydroxy-2-(methylimino)ethyl]phenol + NADH
-
 ?
1.5.1.18 additional information wide range of ethanolamines as substrates, overview Arthrobacter globiformis ?
-
 ?
1.5.1.18 suprifene + NAD+ higher rate than (-)-ephedrine Arthrobacter globiformis p-hydroxy-alpha-[(1-methylimino)ethyl]benzyl alcohol + NADH
-
 ?
1.5.1.18 suprifene + NAD+ suprifene is identical with p-hydroxyephedrine Arthrobacter globiformis p-hydroxy-alpha-[(1-methylimino)ethyl]benzyl alcohol + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.18 25
-
 assay at Arthrobacter globiformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.18 54
-
 2 min, complete loss of activity Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.18 10 11.5 in 0.1 M glycine/NaOH buffer Arthrobacter globiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.18 9.5 12.2 pH 9.5: 60% of maximum activity, pH 12.2: 60% of maximum activity Arthrobacter globiformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.18 6.8
-
 24 h, 68% loss of activity Arthrobacter globiformis
1.5.1.18 7.2
-
 24 h, 53% loss of activity Arthrobacter globiformis
1.5.1.18 7.5 8.5 at 4°C maximum stability, after 24 h 20% loss of activity Arthrobacter globiformis
1.5.1.18 10
-
 24 h, 67% loss of activity Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.18 NAD+ required for enzyme activity, NAD+ can not be replaced by NADP+, FAD or FMN Arthrobacter globiformis