Literature summary extracted from

Smith, G.K.; Banks, S.D.; Monaco, T.J.; Rigual, R.; Duch, D.S.; Mullin, R.J.; Huber, B.E.
Activity of an NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase in normal tissue, neoplastic cells, and oncogene-transformed cells (1990), Arch. Biochem. Biophys., 283, 367-371.

Application

EC Number	Application	Comment	Organism
1.5.1.15	medicine	-	Mus musculus
1.5.1.15	medicine	NAD+ dependent isoenzyme is genuinely tumor-specific, it may provide a novel therapeutic target for cancer therapy and/or an important diagnostic tool	Rattus norvegicus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5.1.15	0.171	-	Mg2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	Mus musculus	-	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	Homo sapiens	-	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	Rattus norvegicus	-	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	Bos taurus	-	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	Rattus norvegicus Fischer	-	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	Mus musculus	-	5,10-methenyl-tetrahydrofolate + NADH	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	Homo sapiens	-	5,10-methenyl-tetrahydrofolate + NADH	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	Rattus norvegicus	-	5,10-methenyl-tetrahydrofolate + NADH	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	Bos taurus	-	5,10-methenyl-tetrahydrofolate + NADH	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	Rattus norvegicus Fischer	-	5,10-methenyl-tetrahydrofolate + NADH	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.1.15	Bos taurus	-	bovine	-
1.5.1.15	Homo sapiens	-	-	-
1.5.1.15	Mus musculus	-	Ehrlich ascites line Swiss mice	-
1.5.1.15	Rattus norvegicus	-	Fischer	-
1.5.1.15	Rattus norvegicus	-	rat, Sprague-Dawley	-
1.5.1.15	Rattus norvegicus Fischer	-	Fischer	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.5.1.15	adrenal cortex	-	Rattus norvegicus	-
1.5.1.15	adrenal cortex	-	Bos taurus	-
1.5.1.15	adrenal medulla	-	Rattus norvegicus	-
1.5.1.15	bone marrow	-	Mus musculus	-
1.5.1.15	bone marrow	-	Rattus norvegicus	-
1.5.1.15	epithelium	-	Rattus norvegicus	-
1.5.1.15	fibroblast	-	Mus musculus	-
1.5.1.15	fibroblast	-	Homo sapiens	-
1.5.1.15	liver	-	Mus musculus	-
1.5.1.15	liver	-	Homo sapiens	-
1.5.1.15	liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.5.1.15	0.0001	-	C-57/Bl, bone marrow and whole adrenal, CD-1, adrenal	Mus musculus
1.5.1.15	0.0002	-	adrenal cortex	Bos taurus
1.5.1.15	0.0002	-	CD-1, spleen	Mus musculus
1.5.1.15	0.0003	-	cell line MRC-5	Homo sapiens
1.5.1.15	0.0003	-	HEP-G2 tumor in nude mice	Homo sapiens
1.5.1.15	0.0003	-	RLE cells and RLE cells virus J5 v-myc oncogene-transformed	Rattus norvegicus
1.5.1.15	0.0004	-	CD-1, bone marrow	Mus musculus
1.5.1.15	0.0005	-	colon 38 solid tumor	Mus musculus
1.5.1.15	0.0005	-	human liver tumor	Homo sapiens
1.5.1.15	0.0006	-	RLE cells, virus 3611.MSV v-raf oncogene-transformed	Rattus norvegicus
1.5.1.15	0.0011	-	Swiss, bone marrow	Mus musculus
1.5.1.15	0.0012	-	cell line Hl-60	Homo sapiens
1.5.1.15	0.0012	-	RLE cells, spontaneous oncogene transformed	Rattus norvegicus
1.5.1.15	0.0014	-	adrenal medulla	Rattus norvegicus
1.5.1.15	0.0015	-	cell line HEP-2	Homo sapiens
1.5.1.15	0.0015	-	RLE cells, aflatoxin oncogene-transformed	Rattus norvegicus
1.5.1.15	0.0017	-	cell line B-16 solid tumor	Mus musculus
1.5.1.15	0.002	-	RLE cells, virus pRNR16 v-Ha-ras oncogene-transformed	Rattus norvegicus
1.5.1.15	0.0021	-	RLE cells, virus J2 v-raf/v-myc oncogene-transformed	Rattus norvegicus
1.5.1.15	0.0027	-	cell line MCF-7, 100% confluent	Homo sapiens
1.5.1.15	0.0028	-	cell line KB	Homo sapiens
1.5.1.15	0.0035	-	cell line MCF-7, 50% confluent	Homo sapiens
1.5.1.15	0.0039	-	cell line P-388 in vitro	Mus musculus
1.5.1.15	0.004	-	cell line CCRF-CEM	Homo sapiens
1.5.1.15	0.0042	-	cell line Y1	Mus musculus
1.5.1.15	0.0045	-	cell line CHO	Mus musculus
1.5.1.15	0.005	-	Ehrlich ascites	Mus musculus
1.5.1.15	0.0052	-	adrenal cortex	Rattus norvegicus
1.5.1.15	0.0058	-	whole adrenal	Rattus norvegicus
1.5.1.15	0.0063	-	cell line B-16 in vitro	Mus musculus
1.5.1.15	0.0063	-	cell line MOLT-4	Homo sapiens
1.5.1.15	0.0071	-	cell line P-388 leukemia ip	Mus musculus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	-	Mus musculus	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	-	Homo sapiens	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	-	Rattus norvegicus	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	-	Bos taurus	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methenyl-tetrahydrofolate + NADH	-	Rattus norvegicus Fischer	L-5,10-methylenetetrahydrofolate + NAD+	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	-	Mus musculus	5,10-methenyl-tetrahydrofolate + NADH	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	-	Homo sapiens	5,10-methenyl-tetrahydrofolate + NADH	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	-	Rattus norvegicus	5,10-methenyl-tetrahydrofolate + NADH	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	-	Bos taurus	5,10-methenyl-tetrahydrofolate + NADH	-	r
1.5.1.15	L-5,10-methylenetetrahydrofolate + NAD+	-	Rattus norvegicus Fischer	5,10-methenyl-tetrahydrofolate + NADH	-	r
1.5.1.15	additional information	-	Homo sapiens	?	-	?
1.5.1.15	additional information	level of enzyme activity appears to be strain dependent with Swiss mice having the highest activity	Mus musculus	?	-	?