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Literature summary extracted from

  • Barlowe, C.K.; Appling, D.R.
    Isolation and characterization of a novel eukaryotic monofunctional NAD+-dependent 5,10-methylenetetrahydrofolate dehydrogenase (1990), Biochemistry, 29, 7089-7094.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.15 0.06
-
 L-5,10-methylenetetrahydrofolate
-
 Saccharomyces cerevisiae
1.5.1.15 1.6
-
 NAD+
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.15 cytoplasm
-
 Homo sapiens 5737
-
1.5.1.15 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
1.5.1.15 cytosol
-
 Homo sapiens 5829
-
1.5.1.15 cytosol
-
 Saccharomyces cerevisiae 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.15 33000 38000 SDS-PAGE Saccharomyces cerevisiae
1.5.1.15 64000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Homo sapiens
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Rattus norvegicus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Clostridium formicaceticum
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Acetobacterium woodii
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae KSY8
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Homo sapiens
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Rattus norvegicus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Clostridium formicaceticum
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Acetobacterium woodii
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae KSY8
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.15 Acetobacterium woodii
-
-
-
1.5.1.15 Clostridium formicaceticum
-
-
-
1.5.1.15 Homo sapiens
-
-
-
1.5.1.15 Rattus norvegicus
-
-
-
1.5.1.15 Saccharomyces cerevisiae
-
-
-
1.5.1.15 Saccharomyces cerevisiae
-
 yeast
-
1.5.1.15 Saccharomyces cerevisiae KSY8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.15
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.15 5.4
-
-
 Saccharomyces cerevisiae
1.5.1.15 27
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.5.1.15 4°C, enzyme activity is stable for several weeks after storage in the final elution buffer Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Homo sapiens L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Rattus norvegicus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Acetobacterium woodii L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae KSY8 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Homo sapiens 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Rattus norvegicus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Clostridium formicaceticum 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Acetobacterium woodii 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae KSY8 5,10-methenyl-tetrahydrofolate + NADH
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.15 8
-
 enzyme activity decreases approximately 2fold at pH 7.0 or pH 9.0 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.15 6 10
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.15 8
-
 enzyme activity decreases approximately 2fold at pH 7.0 or pH 9.0 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.15 NAD+
-
 Homo sapiens
1.5.1.15 NAD+
-
 Saccharomyces cerevisiae
1.5.1.15 NAD+
-
 Clostridium formicaceticum
1.5.1.15 NAD+
-
 Acetobacterium woodii