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Literature summary extracted from

  • Ragsdale, S.W.; Ljungdahl, L.G.
    Purification and properties of NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Acetobacterium woodii (1984), J. Biol. Chem., 259, 3499-3503.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.15 dithiothreitol
-
 Acetobacterium woodii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.15 Zn2+
-
 Acetobacterium woodii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.15 0.26
-
 L-5,10-methylenetetrahydrofolate
-
 Acetobacterium woodii
1.5.1.15 1
-
 5,10-methenyltetrahydrofolate
-
 Acetobacterium woodii
1.5.1.15 2
-
 NADH
-
 Acetobacterium woodii
1.5.1.15 4
-
 NAD+
-
 Acetobacterium woodii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.15 Mg2+ NAD+ dependent enzyme from Ehrlich ascites tumor cells requires Mg2+ or Mn2+ for activity Mus musculus
1.5.1.15 Mn2+ NAD+ dependent enzyme from Ehrlich ascites tumor cells requires Mg2+ or Mn2+ for activity Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.15 26300
-
 2 * 26300, amino acid analysis Acetobacterium woodii
1.5.1.15 26900
-
 2 * 26900, SDS-PAGE Acetobacterium woodii
1.5.1.15 55000
-
 purified enzyme, gel filtration Acetobacterium woodii
1.5.1.15 58000
-
 gel filtration Acetobacterium woodii
1.5.1.15 69000
-
 gel filtration Acetobacterium woodii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Clostridium formicaceticum physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Acetobacterium woodii physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Clostridium formicaceticum
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Acetobacterium woodii
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.15 Acetobacterium woodii
-
-
-
1.5.1.15 Clostridium formicaceticum
-
-
-
1.5.1.15 Mus musculus
-
 mouse, Ehrlich ascites tumor cells
-
1.5.1.15 Saccharomyces cerevisiae
-
 yeast
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.5.1.15 oxygen-labile Acetobacterium woodii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.15
-
 Clostridium formicaceticum
1.5.1.15
-
 Acetobacterium woodii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.15 Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.15 670
-
-
 Acetobacterium woodii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Acetobacterium woodii L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one Acetobacterium woodii L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Clostridium formicaceticum 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Acetobacterium woodii 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.15 dimer
-
 Mus musculus
1.5.1.15 dimer
-
 Saccharomyces cerevisiae
1.5.1.15 dimer
-
 Clostridium formicaceticum
1.5.1.15 dimer 2 * 26300, amino acid analysis Acetobacterium woodii
1.5.1.15 dimer 2 * 26900, SDS-PAGE Acetobacterium woodii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.15 1.58
-
 L-5,10-methenyltetrahydrofolate
-
 Acetobacterium woodii
1.5.1.15 1.63
-
 L-5,10-methylenetetrahydrofolate
-
 Acetobacterium woodii

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.15 NAD+
-
 Mus musculus
1.5.1.15 NAD+
-
 Saccharomyces cerevisiae
1.5.1.15 NAD+
-
 Clostridium formicaceticum
1.5.1.15 NAD+
-
 Acetobacterium woodii