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Literature summary extracted from

  • Forlani, G.; Scainelli, D.; Nielsen, E.
    DELTA1-Pyrroline-5-carboxylate dehydrogenase from cultured cells of potato. Purification and properties (1997), Plant Physiol., 113, 1413-1418.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.88 chloride
-
 Solanum tuberosum
1.2.1.88 sulfate no inhibition Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.88 0.11
-
 NAD+
-
 Solanum tuberosum
1.2.1.88 0.46
-
 1-pyrroline-5-carboxylate
-
 Solanum tuberosum
1.2.1.88 0.662
-
 NADP+
-
 Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.88 60000
-
 4 * 60000, SDS-PAGE Solanum tuberosum
1.2.1.88 240000
-
 gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O Solanum tuberosum
-
 L-glutamate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.88 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.88
-
 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.88 cell culture
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.88 44.15
-
-
 Solanum tuberosum

Storage Stability

EC Number Storage Stability Organism
1.2.1.88 0°C, 0.2 M potsassium phosphate or 0.2 M KCl, pH 7.4, 45 days, 70-80% activity remains Solanum tuberosum
1.2.1.88 0°C, 25 mM Hepes-KOH, pH 7.4, 0.5 mM dithiothreitol, 1 mM MgCl2, 1% polyvinyl polypyrrolidone, loss of 90% activity after 15 days Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Solanum tuberosum L-glutamate + NADH
-
 ?
1.2.1.88 1-pyrroline-5-carboxylate + NADP+ + H2O
-
 Solanum tuberosum L-glutamate + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.88 tetramer 4 * 60000, SDS-PAGE Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
1.2.1.88 P5C dehydrogenase
-
 Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.88 38
-
-
 Solanum tuberosum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.88 15 37.5
-
 Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.88 45
-
 half-life 44 min Solanum tuberosum
1.2.1.88 50
-
 half-life 30 min Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.88 7.3 8.3
-
 Solanum tuberosum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.88 NAD+ preferentially Solanum tuberosum
1.2.1.88 NADP+ 60% of the activity with NAD+ Solanum tuberosum