BRENDA - Enzyme Database show

Ferredoxin-dependent iron-sulfur flavoprotein glutamate synthase (GlsF) from the Cyanobacterium synechocystis sp. PCC 6803: expression and assembly in Escherichia coli

Navarro, F.; Martin-Figueroa, E.; Candau, P.; Florencio, F.J.; Arch. Biochem. Biophys. 379, 267-276 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.4.7.1
expression in Escherichia coli
Synechocystis sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.7.1
2-oxoglutarate
at high concentrations
Synechocystis sp.
1.4.7.1
6-diazo-5-oxo-norleucine
0.05 mM, 99% inhibition
Synechocystis sp.
1.4.7.1
azaserine
0.05 mM, 62% inhibition
Synechocystis sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.7.1
0.0035
-
Ferredoxin
native enzyme
Synechocystis sp.
1.4.7.1
0.0045
-
Ferredoxin
recombinant enzyme
Synechocystis sp.
1.4.7.1
0.5
-
2-oxoglutarate
native enzyme
Synechocystis sp.
1.4.7.1
0.6
-
2-oxoglutarate
recombinant enzyme
Synechocystis sp.
1.4.7.1
2.2
-
L-glutamine
recombinant enzyme
Synechocystis sp.
1.4.7.1
2.5
-
L-glutamine
native enzyme
Synechocystis sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.7.1
Iron
enzyme contains a single [3Fe-4S] cluster
Synechocystis sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.7.1
175000
-
recombinant enzyme, gel filtration
Synechocystis sp.
1.4.7.1
178000
-
native enzyme, gel filtration
Synechocystis sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
Synechocystis sp.
-
L-glutamate + oxidized ferredoxin
-
Synechocystis sp.
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.7.1
Synechocystis sp.
-
PCC 6803, cyanobacterium
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.7.1
recombinant enzyme, affinity chromatography on ferredoxin-Sepharose, native enzyme, partially purified
Synechocystis sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.4.7.1
36.6
-
recombinant enzyme
Synechocystis sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
392028
Synechocystis sp.
L-glutamate + oxidized ferredoxin
-
392028
Synechocystis sp.
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced methylviologen
-
392028
Synechocystis sp.
L-glutamate + oxidized methylviologen
-
392028
Synechocystis sp.
?
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
1.4.7.1
55
-
50% activity remains after 20 min, recombinant enzyme
Synechocystis sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.7.1
FMN
one FMN per enzyme molecule
Synechocystis sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.7.1
expression in Escherichia coli
Synechocystis sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.7.1
FMN
one FMN per enzyme molecule
Synechocystis sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.7.1
2-oxoglutarate
at high concentrations
Synechocystis sp.
1.4.7.1
6-diazo-5-oxo-norleucine
0.05 mM, 99% inhibition
Synechocystis sp.
1.4.7.1
azaserine
0.05 mM, 62% inhibition
Synechocystis sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.7.1
0.0035
-
Ferredoxin
native enzyme
Synechocystis sp.
1.4.7.1
0.0045
-
Ferredoxin
recombinant enzyme
Synechocystis sp.
1.4.7.1
0.5
-
2-oxoglutarate
native enzyme
Synechocystis sp.
1.4.7.1
0.6
-
2-oxoglutarate
recombinant enzyme
Synechocystis sp.
1.4.7.1
2.2
-
L-glutamine
recombinant enzyme
Synechocystis sp.
1.4.7.1
2.5
-
L-glutamine
native enzyme
Synechocystis sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.7.1
Iron
enzyme contains a single [3Fe-4S] cluster
Synechocystis sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.7.1
175000
-
recombinant enzyme, gel filtration
Synechocystis sp.
1.4.7.1
178000
-
native enzyme, gel filtration
Synechocystis sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
Synechocystis sp.
-
L-glutamate + oxidized ferredoxin
-
Synechocystis sp.
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.7.1
recombinant enzyme, affinity chromatography on ferredoxin-Sepharose, native enzyme, partially purified
Synechocystis sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.4.7.1
36.6
-
recombinant enzyme
Synechocystis sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
392028
Synechocystis sp.
L-glutamate + oxidized ferredoxin
-
392028
Synechocystis sp.
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced methylviologen
-
392028
Synechocystis sp.
L-glutamate + oxidized methylviologen
-
392028
Synechocystis sp.
?
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
1.4.7.1
55
-
50% activity remains after 20 min, recombinant enzyme
Synechocystis sp.