BRENDA - Enzyme Database show

Glutamate synthase from rice leaves

Suzuki, A.; Gadal, P.; Plant Physiol. 69, 848-852 (1982)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.7.1
azaserine
-
Oryza sativa
1.4.7.1
Cd2+
5 mM, 69% inhibition
Oryza sativa
1.4.7.1
Co2+
5 mM, 65% inhibition
Oryza sativa
1.4.7.1
Mn2+
5 mM, 45% inhibition
Oryza sativa
1.4.7.1
Ni2+
5 mM, 73% inhibition
Oryza sativa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.7.1
0.0055
-
Ferredoxin
-
Oryza sativa
1.4.7.1
0.33
-
2-oxoglutarate
-
Oryza sativa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.4.7.1
chloroplast
-
Oryza sativa
9507
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.7.1
Ca2+
1 mM, 6% activation
Oryza sativa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.7.1
115000
-
2 * 115000, SDS-PAGE
Oryza sativa
1.4.7.1
224000
-
gel filtration
Oryza sativa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
Oryza sativa
-
L-glutamate + oxidized ferredoxin
-
Oryza sativa
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.7.1
Oryza sativa
-
rice
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.7.1
affinity chromatography on ferredoxin Sepharose
Oryza sativa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.7.1
leaf
-
Oryza sativa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.7.1
35.88
-
-
Oryza sativa
1.4.7.1
35.9
-
-
Oryza sativa
Storage Stability
EC Number
Storage Stability
Organism
1.4.7.1
-20°C, 5 mM dithiothreitol, 20 days, 54% loss of activity
Oryza sativa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
392018
Oryza sativa
L-glutamate + oxidized ferredoxin
-
392018
Oryza sativa
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced methylviologen
artificial elctron donor
392018
Oryza sativa
L-glutamate + oxidized methylviologen
-
392018
Oryza sativa
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.7.1
dimer
2 * 115000, SDS-PAGE
Oryza sativa
1.4.7.1
monomer
SDS-PAGE
Oryza sativa
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.7.1
137
-
L-glutamine
-
Oryza sativa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.7.1
7.3
-
-
Oryza sativa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.7.1
azaserine
-
Oryza sativa
1.4.7.1
Cd2+
5 mM, 69% inhibition
Oryza sativa
1.4.7.1
Co2+
5 mM, 65% inhibition
Oryza sativa
1.4.7.1
Mn2+
5 mM, 45% inhibition
Oryza sativa
1.4.7.1
Ni2+
5 mM, 73% inhibition
Oryza sativa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.7.1
0.0055
-
Ferredoxin
-
Oryza sativa
1.4.7.1
0.33
-
2-oxoglutarate
-
Oryza sativa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.4.7.1
chloroplast
-
Oryza sativa
9507
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.7.1
Ca2+
1 mM, 6% activation
Oryza sativa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.7.1
115000
-
2 * 115000, SDS-PAGE
Oryza sativa
1.4.7.1
224000
-
gel filtration
Oryza sativa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
Oryza sativa
-
L-glutamate + oxidized ferredoxin
-
Oryza sativa
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.7.1
affinity chromatography on ferredoxin Sepharose
Oryza sativa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.7.1
leaf
-
Oryza sativa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.7.1
35.88
-
-
Oryza sativa
1.4.7.1
35.9
-
-
Oryza sativa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.4.7.1
-20°C, 5 mM dithiothreitol, 20 days, 54% loss of activity
Oryza sativa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
392018
Oryza sativa
L-glutamate + oxidized ferredoxin
-
392018
Oryza sativa
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced methylviologen
artificial elctron donor
392018
Oryza sativa
L-glutamate + oxidized methylviologen
-
392018
Oryza sativa
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.7.1
dimer
2 * 115000, SDS-PAGE
Oryza sativa
1.4.7.1
monomer
SDS-PAGE
Oryza sativa
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.7.1
137
-
L-glutamine
-
Oryza sativa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.7.1
7.3
-
-
Oryza sativa