BRENDA - Enzyme Database

Lentil seedlings amine oxidase: preparation and properties of the copper-free enzyme

Matsumiya, M.; Otake, S.; Nippon Suisan Gakkaishi 52, 1617-1623 (1986)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
2-mercaptoethanol
0.1 mM, 89% inhibition
Seriola quinqueradiata
1.4.3.22
aminoguanidine
0.03 mM, complete inhibition
Seriola quinqueradiata
1.4.3.22
Cu2+
0.5 mM, 92% inhibition
Seriola quinqueradiata
1.4.3.22
Hg2+
0.5 mM, complete inactivation
Seriola quinqueradiata
1.4.3.22
Iproniazid
0.03 mM, 84% inhibition
Seriola quinqueradiata
1.4.3.22
KMnO4
0.1 mM, complete inhibition
Seriola quinqueradiata
1.4.3.22
Ni2+
0.5 mM, 92% inhibition
Seriola quinqueradiata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.22
0.0146
-
cadaverine
-
Seriola quinqueradiata
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.22
Al3+
activates
Seriola quinqueradiata
1.4.3.22
Co2+
activates
Seriola quinqueradiata
1.4.3.22
Manganese
Mn2+ activates
Seriola quinqueradiata
1.4.3.22
Zn2+
activates
Seriola quinqueradiata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
60000
-
1 * 80000 + 1 * 60000, SDS-PAGE
Seriola quinqueradiata
1.4.3.22
80000
-
1 * 80000 + 1 * 60000, SDS-PAGE
Seriola quinqueradiata
1.4.3.22
140000
-
gel filtration
Seriola quinqueradiata
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.22
Seriola quinqueradiata
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.22
-
Seriola quinqueradiata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.22
cecum
-
Seriola quinqueradiata
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.4.3.22
21.1
-
-
Seriola quinqueradiata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1,3-diaminopropane + H2O + O2
-
391945
Seriola quinqueradiata
3-aminopropanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391945
Seriola quinqueradiata
4-aminobutanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
1,5-diaminopentane + H2O + O2
-
391945
Seriola quinqueradiata
5-aminopentanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
1,6-diaminohexane + H2O + O2
-
391945
Seriola quinqueradiata
6-aminohexanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
-
1.4.3.22
1,7-diaminoheptane + H2O + O2
-
391945
Seriola quinqueradiata
7-aminoheptanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
1,8-diaminooctane + H2O + O2
-
391945
Seriola quinqueradiata
8-aminooctanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
agmatine
-
391945
Seriola quinqueradiata
?
-
-
-
?
1.4.3.22
histamine + H2O + O2
-
391945
Seriola quinqueradiata
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391945
Seriola quinqueradiata
RCHO + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
spermidine + H2O + O2
-
391945
Seriola quinqueradiata
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.3.22
dimer
1 * 80000 + 1 * 60000, SDS-PAGE
Seriola quinqueradiata
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.4.3.22
50
-
25% activity at 20C
Seriola quinqueradiata
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.4.3.22
40
-
stable below, unstable above 50C
Seriola quinqueradiata
1.4.3.22
60
-
activity is completely lost after 30 min
Seriola quinqueradiata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
7
-
histamine
Seriola quinqueradiata
1.4.3.22
7.5
-
cadaverine
Seriola quinqueradiata
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.22
6
-
unstable below
Seriola quinqueradiata
1.4.3.22
7
8
extremely unstable below pH 6.0 and above pH 9.0
Seriola quinqueradiata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
2-mercaptoethanol
0.1 mM, 89% inhibition
Seriola quinqueradiata
1.4.3.22
aminoguanidine
0.03 mM, complete inhibition
Seriola quinqueradiata
1.4.3.22
Cu2+
0.5 mM, 92% inhibition
Seriola quinqueradiata
1.4.3.22
Hg2+
0.5 mM, complete inactivation
Seriola quinqueradiata
1.4.3.22
Iproniazid
0.03 mM, 84% inhibition
Seriola quinqueradiata
1.4.3.22
KMnO4
0.1 mM, complete inhibition
Seriola quinqueradiata
1.4.3.22
Ni2+
0.5 mM, 92% inhibition
Seriola quinqueradiata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.22
0.0146
-
cadaverine
-
Seriola quinqueradiata
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.22
Al3+
activates
Seriola quinqueradiata
1.4.3.22
Co2+
activates
Seriola quinqueradiata
1.4.3.22
Manganese
Mn2+ activates
Seriola quinqueradiata
1.4.3.22
Zn2+
activates
Seriola quinqueradiata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
60000
-
1 * 80000 + 1 * 60000, SDS-PAGE
Seriola quinqueradiata
1.4.3.22
80000
-
1 * 80000 + 1 * 60000, SDS-PAGE
Seriola quinqueradiata
1.4.3.22
140000
-
gel filtration
Seriola quinqueradiata
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.22
-
Seriola quinqueradiata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.22
cecum
-
Seriola quinqueradiata
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.4.3.22
21.1
-
-
Seriola quinqueradiata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1,3-diaminopropane + H2O + O2
-
391945
Seriola quinqueradiata
3-aminopropanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391945
Seriola quinqueradiata
4-aminobutanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
1,5-diaminopentane + H2O + O2
-
391945
Seriola quinqueradiata
5-aminopentanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
1,6-diaminohexane + H2O + O2
-
391945
Seriola quinqueradiata
6-aminohexanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
-
1.4.3.22
1,7-diaminoheptane + H2O + O2
-
391945
Seriola quinqueradiata
7-aminoheptanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
1,8-diaminooctane + H2O + O2
-
391945
Seriola quinqueradiata
8-aminooctanal + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
agmatine
-
391945
Seriola quinqueradiata
?
-
-
-
?
1.4.3.22
histamine + H2O + O2
-
391945
Seriola quinqueradiata
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391945
Seriola quinqueradiata
RCHO + NH3 + H2O2
-
391945
Seriola quinqueradiata
?
1.4.3.22
spermidine + H2O + O2
-
391945
Seriola quinqueradiata
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.3.22
dimer
1 * 80000 + 1 * 60000, SDS-PAGE
Seriola quinqueradiata
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.4.3.22
50
-
25% activity at 20C
Seriola quinqueradiata
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.4.3.22
40
-
stable below, unstable above 50C
Seriola quinqueradiata
1.4.3.22
60
-
activity is completely lost after 30 min
Seriola quinqueradiata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
7
-
histamine
Seriola quinqueradiata
1.4.3.22
7.5
-
cadaverine
Seriola quinqueradiata
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.22
6
-
unstable below
Seriola quinqueradiata
1.4.3.22
7
8
extremely unstable below pH 6.0 and above pH 9.0
Seriola quinqueradiata