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Literature summary extracted from

  • Zhao, G.; Winkler, M.E.
    Kinetic limitation and cellular amount of pyridoxine (pyridoxamine) 5'-phosphate oxidase of Escherichia coli K-12 (1995), J. Bacteriol., 177, 883-891.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.5 expression in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.5 4-deoxypyridoxine 5'-phosphate competitive inhibition Escherichia coli
1.4.3.5 pyridoxal 5'-phosphate competitive inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.002
-
 pyridoxine 5'-phosphate recombinant enzyme Escherichia coli
1.4.3.5 0.105
-
 pyridoxamine 5'-phosphate recombinant enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.5 28000
-
 2 * 28000, SDS-PAGE Escherichia coli
1.4.3.5 51000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 recombinant enzyme, DEAE-cellulose, hydroxyapatite, CM-cellulose, Bio-Gel Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 1.37
-
 recombinant enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.4.3.5 -75°C, 15% glycerol, several months, no loss in activity Escherichia coli
1.4.3.5 4°C, several weeks, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Escherichia coli pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.5 dimer 2 * 28000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.5 50
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.5 65
-
 no loss in activity after incubation at temperatures below 50°C, 30% to 40% activity loss after 15 min at 65°C Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.5 0.76
-
 pyridoxine 5'-phosphate recombinant enzyme Escherichia coli
1.4.3.5 1.72
-
 pyridoxamine 5'-phosphate recombinant enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.5 8.5
-
 pyridoxine phosphate oxidase activity, slight optimum Escherichia coli
1.4.3.5 8.5 10 pyridoxamine phosphate oxidase activity remains nearly constant between pH 8.5 and 10.0 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.5 6 10 pyridoxine phosphate oxidase activity Escherichia coli
1.4.3.5 6.5 10 pyridoxamine phosphate oxidase activity is absent at pH 6.0, rises sharply from pH 6.0 to 8.5 and remains constantly high between pH 8.5 and 10.0 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.5 0.008
-
 pyridoxal 5'-phosphate
-
 Escherichia coli
1.4.3.5 0.105
-
 4'-deoxypyridoxine-5'-phosphate
-
 Escherichia coli