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Literature summary extracted from

  • Horiike, K.; Merrill, A.H.; McCormick, D.B.
    Activation and inactivation of rabbit liver pyridoxamine (pyridoxine) 5-phosphate oxidase activity by urea and other solutes (1979), Arch. Biochem. Biophys., 195, 325-335.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.5 guanidine hydrochloride 500 mM, activation at low concentrations and brief periods of exposure, inactivation at higher concentrations and longer periods of exposure Oryctolagus cuniculus
1.4.3.5 guanidine hydrochloride urea both activates and denatures the enzyme Oryctolagus cuniculus
1.4.3.5 Urea 2.0-2.5 M, rapid and reversible activation at low concentrations and brief periods of exposure, inactivation at higher concentrations and longer periods of exposure Oryctolagus cuniculus
1.4.3.5 Urea both activates and denatures the enzyme, rapid and reversible activation at 2.0-2.5 M urea Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.5 guanidine hydrochloride urea both activates and denatures the enzyme Oryctolagus cuniculus
1.4.3.5 pyridoxal 5'-phosphate potent product inhibition Oryctolagus cuniculus
1.4.3.5 Urea both activates and denatures the enzyme, reversible inactivation above 2.5 M, half-time of inactivation is 46 min at 2 M and 37°C Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 additional information
-
 additional information steady-state kinetics Oryctolagus cuniculus
1.4.3.5 0.0112
-
 pyridoxamine 5'-phosphate
-
 Oryctolagus cuniculus
1.4.3.5 0.0112
-
 pyridoxamine 5'-phosphate pH 8.0, 37°C Oryctolagus cuniculus
1.4.3.5 0.0187
-
 pyridoxamine 5'-phosphate in the presence of 1 M urea Oryctolagus cuniculus
1.4.3.5 0.0187
-
 pyridoxamine 5'-phosphate pH 8.0, 37°C, in presence of 1 M urea Oryctolagus cuniculus
1.4.3.5 0.0238
-
 pyridoxine 5'-phosphate
-
 Oryctolagus cuniculus
1.4.3.5 0.0238
-
 pyridoxine 5'-phosphate pH 8.0, 37°C Oryctolagus cuniculus
1.4.3.5 0.0417
-
 pyridoxamine 5'-phosphate in the presence of 2 M urea Oryctolagus cuniculus
1.4.3.5 0.0417
-
 pyridoxamine 5'-phosphate pH 8.0, 37°C, in presence of 2 M urea Oryctolagus cuniculus
1.4.3.5 0.0667
-
 pyridoxine 5'-phosphate in the presence of 2 M urea Oryctolagus cuniculus
1.4.3.5 0.0667
-
 pyridoxine 5'-phosphate pH 8.0, 37°C, in presence of 2 M urea Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.5 additional information neutral salts, such as LiCl, KCl, NaCl, KBr, KI, and KSCN, both activate and denature the enzyme Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.5 27000 28000 gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 native enzyme from liver Oryctolagus cuniculus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.4.3.5 urea both activates and denatures the enzyme, reversible inactivation above 2.5 M, half-time of inactivation is 46 min at 2 M and 37°C Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.5 liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + FMN + H2O + 2 H+
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + FMNH2 + NH3
-
 ?
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + FMN
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + FMNH2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.5 monomer 1 * 27000-28000, denaturing gel filtration Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 pyridoxamine (pyridoxine) 5'-phosphate oxidase
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.5 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.5 8
-
 assay at Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FMN bound, required Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.5 additional information
-
 additional information inhibition kinetics of urea, guanidinium hydrochloride, and neutral salts such as LiCl, KCl, NaCl, KBr, KI, and KSCN Oryctolagus cuniculus
1.4.3.5 0.019
-
 pyridoxal 5'-phosphate pH 8.0, 37°C Oryctolagus cuniculus
1.4.3.5 0.025
-
 pyridoxal 5'-phosphate pH 8.0, 37°C, in presence of 1 M urea Oryctolagus cuniculus
1.4.3.5 0.044
-
 pyridoxal 5'-phosphate pH 8.0, 37°C, in presence of 2 M urea Oryctolagus cuniculus