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Literature summary extracted from
- Trichomonas vaginalis: characterization of its glutamate dehydrogenase (1988), Exp. Parasitol., 67, 47-53.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.4 | GDP | at 1 mM five-fold Michaelis Menten constant of 2-oxoglutarate | Trichomonas vaginalis |  |
| 1.4.1.4 | IDP | at 1 mM five-fold Michaelis Menten constant of 2-oxoglutarate | Trichomonas vaginalis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.4 | 2-oxoglutarate | competitive inhibitor of the deamination | Trichomonas vaginalis | |
| 1.4.1.4 | glutamate | competitive inhibitor of the amination reaction | Trichomonas vaginalis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.4 | 0.01 | - |
NADPH | reductive amination | Trichomonas vaginalis | |
| 1.4.1.4 | 0.04 | - |
NADP+ | oxidative deamination | Trichomonas vaginalis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.4.1.4 | soluble | - |
Trichomonas vaginalis | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 230000 | - |
gel filtration | Trichomonas vaginalis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.4 | L-glutamate + NADP+ + H2O | Trichomonas vaginalis | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.4 | Trichomonas vaginalis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.4 | partially | Trichomonas vaginalis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 133 | - |
reductive amination at 37°C and pH 6.7 | Trichomonas vaginalis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.4 | L-glutamate + NADP+ + H2O | - |
Trichomonas vaginalis | 2-oxoglutarate + NADPH + NH3 | - |
r | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 57 | - |
after 1 h stable | Trichomonas vaginalis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 6.7 | - |
reductive amination | Trichomonas vaginalis |
| 1.4.1.4 | 8 | - |
oxidative deamination | Trichomonas vaginalis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.4 | NADP+ | - |
Trichomonas vaginalis | |
| 1.4.1.4 | NADPH | - |
Trichomonas vaginalis | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.4 | 0.45 | - |
2-oxoglutarate | competitive | Trichomonas vaginalis | |
| 1.4.1.4 | 5.6 | - |
glutamate | competitive inhibitor | Trichomonas vaginalis | |
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