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Literature summary extracted from

  • Robb, F.T.; Maeder, D.L.; DiRuggiero, J.; Borges, K.M.; Tolliday, N.
    Glutamate dehydrogenases from hyperthermophiles (2001), Methods Enzymol., 331, 26-41.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.4 expression in Escherichia coli BL21 Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.00056
-
 NH3 recombinant enzyme, reductive amination Thermococcus litoralis
1.4.1.4 0.00065
-
 NH3 wild-type enzyme, reductive amination Thermococcus litoralis
1.4.1.4 0.0098
-
 NADP+ recombinant enzyme, oxidative deamination Thermococcus litoralis
1.4.1.4 0.0102
-
 NADP+ wild-type enzyme, oxidative deamination Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 45000
-
 alpha6, 6 * 45000, SDS-PAGE, expressed in Escherichia coli Thermococcus litoralis
1.4.1.4 47000
-
 x * 47000, gel filtration Thermococcus litoralis
1.4.1.4 47169
-
 x * 47169, amino acid analysis Thermococcus litoralis
1.4.1.4 270000
-
 gel filtration, expressed in Escherichia coli Thermococcus litoralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Thermococcus litoralis
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 additional information Thermococcus litoralis glutamate dehydrogenase represents an enzymatic link between major catabolic and biosynthetic pathways via the tricarboxylic acid cycle intermediate 2-oxoglutarate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Thermococcus litoralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 194.3
-
 oxidative deamination at 75°C Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Thermococcus litoralis 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 additional information glutamate dehydrogenase represents an enzymatic link between major catabolic and biosynthetic pathways via the tricarboxylic acid cycle intermediate 2-oxoglutarate Thermococcus litoralis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.4 ? x * 47000, gel filtration Thermococcus litoralis
1.4.1.4 ? x * 47169, amino acid analysis Thermococcus litoralis
1.4.1.4 homohexamer alpha6, 6 * 45000, SDS-PAGE, expressed in Escherichia coli Thermococcus litoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 85
-
 wild-type enzyme retains 100% activity after 3 h of incubation Thermococcus litoralis
1.4.1.4 85
-
 recombinant enzyme retains 90% activity after 3 h of incubation Thermococcus litoralis
1.4.1.4 98
-
 recombinant enzyme retains 50% activity after 1.5 h of incubation Thermococcus litoralis
1.4.1.4 98
-
 wild-type enzyme retains 50% activity after 6.4 h of incubation Thermococcus litoralis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Thermococcus litoralis
1.4.1.4 NADPH
-
 Thermococcus litoralis