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Literature summary extracted from

  • Jahns, T.; Kaltwasser, H.
    Properties of the cold-labile NAD+-specific glutamate dehydrogenase from Bacillus cereus DSM 31 (1993), J. Gen. Microbiol., 139, 775-780.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.2 additional information no inhibition or activation in the presence of 500 mM AMP, ADP, ATP, cyclic-AMP, GMP, GDP or GTP Bacillus cereus

General Stability

EC Number General Stability Organism
1.4.1.2 extremely unstable at 0°C to 4°C due to the dissociation of the holoenzyme into catalytically inactive subunits Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.2 additional information no inhibition or activation in the presence of 500 mM AMP, ADP, ATP, cyclic-AMP, GMP, GDP or GTP Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.2 0.033
-
 NH4+ for 200 mM NH4+ Bacillus cereus
1.4.1.2 0.56
-
 NAD+
-
 Bacillus cereus
1.4.1.2 7.4
-
 glutamate
-
 Bacillus cereus
1.4.1.2 96
-
 NH4+ from double-reciprocal plots for concentrations between 5 mM and 200 mM Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.2 270000
-
 gel filtration Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Bacillus cereus
-
 strain DSM 31
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2 18-22°C, 20% glycerol, heat treatment, ammonium sulfate, gel filtration, ion-exchange chromatography Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.2 286.4
-
-
 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 2-oxoglutarate + NH4+ + NADPH
-
 Bacillus cereus L-glutamate + NADP+
-
 ?
1.4.1.2 L-glutamate + H2O + NAD(P)+
-
 Bacillus cereus 2-oxoglutarate + NH3 + NAD(P)H
-
 r
1.4.1.2 L-glutamate + H2O + NAD+
-
 Bacillus cereus 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.2
-
-
-
 Bacillus cereus
1.4.1.2 20 25 no activity in cell extracts prepared at 0 to 4°C, high activities between 20°C and 25°C, 70% activity is retained after 2 h at 0°C in the presence of 20% glycerol Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.2 7.7
-
 reductive amination of 2-oxoglutarate Bacillus cereus
1.4.1.2 9.4
-
 oxidative deamination of glutamate Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+ no activity with NADP+ Bacillus cereus