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Literature summary extracted from

  • Veronese, F.M.; Bevilacqua, R.; Boccu, E.; Brown, D.M.
    Purification, characteristics and sequence of a peptide containing an essential lysine residue (1976), Biochim. Biophys. Acta, 445, 1-13.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.3 ADP stimulation Thunnus thynnus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.3
-
 Thunnus thynnus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.3 GTP
-
 Thunnus thynnus
1.4.1.3 isophthalate competitive vs. glutamate Thunnus thynnus
1.4.1.3 pyridoxal 5'-phosphate 0.11 mM, approx. 30% inactivation after 10 min, 0.78 mM, 80% inactivation, inactivation is completely reversed by dialysis Thunnus thynnus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 53900
-
 6 * 53900, sedimentation equilibrium of enzyme treated with 6 M guanidinium and 0.5% 2-mercaptoethanol Thunnus thynnus
1.4.1.3 333000
-
-
 Thunnus thynnus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Thunnus thynnus
-
 tuna
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3
-
 Thunnus thynnus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.3 liver
-
 Thunnus thynnus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 1.93
-
 after 3rd crystallization Thunnus thynnus

Storage Stability

EC Number Storage Stability Organism
1.4.1.3 suspended in ammonium sulfate, stable for months Thunnus thynnus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Thunnus thynnus 2-oxoglutarate + NH3 + NAD(P)H
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.3 hexamer 6 * 53900, sedimentation equilibrium of enzyme treated with 6 M guanidinium and 0.5% 2-mercaptoethanol Thunnus thynnus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.3 25
-
 glutamate oxidation Thunnus thynnus
1.4.1.3 510
-
 2-oxoglutarate reduction Thunnus thynnus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.3 NADH reaction rate NADH/NADPH: 15 Thunnus thynnus
1.4.1.3 NADPH reaction rate NADH/NADPH: 15 Thunnus thynnus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.3 0.28
-
 isophthalate
-
 Thunnus thynnus