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Literature summary extracted from

  • Garland, W.J.; Dennis, D.T.
    Steady-state kinetics of glutamate dehydrogenase from Pisum sativum L. mitochondria (1977), Arch. Biochem. Biophys., 182, 614-625.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.2 alpha-ketoglutarate
-
 Pisum sativum
1.4.1.2 Ca2+ activation of reductive amination; slight inhibition of oxidative deamination Pisum sativum
1.4.1.2 NAD+
-
 Pisum sativum
1.4.1.2 NADH
-
 Pisum sativum
1.4.1.2 NH4+
-
 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.1.2 mitochondrion
-
 Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.2 Ca2+ activation of reductive amination Pisum sativum
1.4.1.2 Ca2+ slight inhibition of oxidative deamination Pisum sativum
1.4.1.2 Mn2+ activation of reductive amination Pisum sativum
1.4.1.2 Zn2+ activation of reductive amination Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2
-
 Pisum sativum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.1.2 L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH + H+ partially random mechanism Pisum sativum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.2 stem
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.2 54
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+
-
 Pisum sativum 2-oxoglutarate + NH3 + NADH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.2 8
-
 reductive amination Pisum sativum
1.4.1.2 9.2
-
 oxidative deamination Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.2 7 8.8 about 50% of activity maximum at pH 7.0 and 8.8, reductive amination Pisum sativum
1.4.1.2 8.4 10.1 50% of activity maximum at pH 8.4 and 10.1, oxidative deamination Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+
-
 Pisum sativum
1.4.1.2 NADH
-
 Pisum sativum