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Literature summary extracted from

  • Ishino, S.; Mizukami, T.; Yamaguchi, K.; Katsumata, R.; Araki, K.
    Cloning and sequencing of the meso-diaminopimelate-D-dehydrogenase (ddh) gene of Corynebacterium glutamicum (1988), Agric. Biol. Chem., 52, 2903-2909.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.16 expression in Escherichia coli and Corynebacterim glutamicum Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.16 35099
-
 2 * 35099, nucleotide sequencing Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Corynebacterium glutamicum involved in lysine biosynthesis meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Corynebacterium glutamicum RRL-5 involved in lysine biosynthesis meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Corynebacterium glutamicum
-
-
-
1.4.1.16 Corynebacterium glutamicum RRL-5
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.16 0.16
-
 activity in strain RRL-5 Corynebacterium glutamicum
1.4.1.16 2.22
-
 activity in strain RRL-5 transformed with plasmid harboring cloned gene Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Corynebacterium glutamicum meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Corynebacterium glutamicum RRL-5 meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH involved in lysine biosynthesis Corynebacterium glutamicum meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH involved in lysine biosynthesis Corynebacterium glutamicum RRL-5 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.16 dimer 2 * 35099, nucleotide sequencing Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.16 NADPH
-
 Corynebacterium glutamicum