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Literature summary extracted from

  • Rendina, A.R.; Orme-Johnson, W.H.
    Glutamate synthase: on the kinetic mechanism of the enzyme from Escherichia coli W (1978), Biochemistry, 17, 5388-5393.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 2'-adenylic acid competitive inhibitor of the forward reaction with NADPH as the varied substrate, uncompetitive inhibitor with alpha-ketoglutarate or L-glutamine Escherichia coli
1.4.1.13 L-glutamate competitive inhibitor of the forward reaction with L-glutamine as the varied substrate, noncompetitive inhibitor with alpha-ketoglutarate as the varied substrate Escherichia coli
1.4.1.13 L-methionine sulfone competitive inhibitor vs. L-glutamine, noncompetitive inhibitor vs. alpha-ketoglutarate and uncompetitive vs. NADPH Escherichia coli
1.4.1.13 NADP+ competitive vs. NADPH and noncompetitive vs. L-glutamine Escherichia coli
1.4.1.13 O-carbamoylserine competitive inhibitor vs. L-glutamine Escherichia coli
1.4.1.13 oxalylglycine competitive inhibitor vs. alpha-ketoglutarate, noncompetitive inhibitor vs. L-glutamine and uncompetitive vs. NADPH Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.0022
-
 NADPH in the presence of 1 mM alpha-ketoglutarate and 5 mM L-glutamine Escherichia coli
1.4.1.13 0.0047
-
 alpha-ketoglutarate in the presence of 0.04 mM NADPH and 5 mM L-glutamine Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.1.13 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+ kinetic mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+
-
 Escherichia coli L-glutamate + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.13 7.5
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.13 additional information
-
 additional information
-
 Escherichia coli