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Literature summary extracted from

  • Kuchler, B.; Abdel-Ghany, A.G.; Bross, P.; Nandy, A.; Rasched, I.; Ghisla, S.
    Biochemical characterization of a variant human medium-chain acyl-CoA dehydrogenase with a disease-associated mutation localized in the active site (1999), Biochem. J., 337, 225-230.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.7 expression of T168A mutant enzyme in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.7 T168A thermostability is markedly decreased, 80% lowered activity in ferricinum assay Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.7 Homo sapiens
-
 T168A mutation found in patients with medium-chain acyl-CoA deficiency
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.7 recombinant T168A mutant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.7 octanoyl-CoA + recombinant beta-Y16L electron transferring flavoprotein
-
 Homo sapiens trans-oct-2-enoyl-CoA + reduced recombinant beta-Y16L electron transferring flavoprotein
-
 r
1.3.8.7 octanoyl-CoA + recombinant electron transferring flavoprotein
-
 Homo sapiens trans-oct-2-enoyl-CoA + reduced recombinant electron transferring flavoprotein
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.8.7 41
-
 wild-type enzyme: almost no loss of activity after 1 h at 41°C, T168A mutant enzyme: complete inactivation after 20 min at 41°C Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.7 1
-
 recombinant electron transferring flavoprotein T168A mutant enzyme Homo sapiens
1.3.8.7 1.67
-
 recombinant beta-Y16L electron transferring flavoprotein T168A mutant enzyme Homo sapiens
1.3.8.7 14.7
-
 recombinant electron transferring flavoprotein wild-type enzyme Homo sapiens
1.3.8.7 18.3
-
 recombinant beta-Y16L electron transferring flavoprotein wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.7 9 10 T168A mutant Homo sapiens
1.3.8.7 10
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.8.7 6 10 T168A mutant enzyme, approx. 20% of maximum activity at pH 6.0, sigmoidal increase between pH 5.5 and pH 9.0, approx. 80% of maximal activity at pH 7.8 Homo sapiens
1.3.8.7 7 10 approx. 20% of maximum activity at pH 7.5, sigmoidal increase between pH 7.0 and pH 10.0, approx. 80% of maximal activity at pH 9.0 Homo sapiens