BRENDA - Enzyme Database

Purification and properties of acyl coenzyme A dehydrogenases from bovine liver. Formation of 2-trans,4-cis-decadienoyl coenzyme A

Dommes, V.; Kunau, W.H.; J. Biol. Chem. 259, 1789-1797 (1984)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.8.1
0.009
-
butyryl-CoA
-
Bos taurus
1.3.8.1
0.02
-
hexanoyl-CoA
-
Bos taurus
1.3.8.1
0.032
-
octanoyl-CoA
-
Bos taurus
1.3.8.7
0.002
-
all-cis-4,7,10,13-hexadecatetraenoyl-CoA
-
Bos taurus
1.3.8.7
0.0021
-
6-cis-decenoyl-CoA
-
Bos taurus
1.3.8.7
0.0023
-
4-cis,7-cis-decadienoyl-CoA
-
Bos taurus
1.3.8.7
0.0029
-
decanoyl-CoA
-
Bos taurus
1.3.8.7
0.0029
-
4-decynoyl-CoA
-
Bos taurus
1.3.8.7
0.003
-
5-cis-decenoyl-CoA
-
Bos taurus
1.3.8.7
0.0032
-
octanoyl-CoA
-
Bos taurus
1.3.8.7
0.004
-
hexanoyl-CoA
-
Bos taurus
1.3.8.7
0.004
-
4-cis-decenoyl-CoA
-
Bos taurus
1.3.8.7
0.0043
-
dodecanoyl-CoA
-
Bos taurus
1.3.8.7
0.005
-
hexanoyl-CoA
-
Bos taurus
1.3.8.7
0.0057
-
tetradecanoyl-CoA
-
Bos taurus
1.3.8.7
0.0084
-
butanoyl-CoA
-
Bos taurus
1.3.8.8
0.13
-
decanoyl-CoA
-
Bos taurus
1.3.8.8
0.2
-
dodecanoyl-CoA
-
Bos taurus
1.3.8.8
0.2
-
5-cis-decenoyl-CoA
-
Bos taurus
1.3.8.8
0.23
-
octanoyl-CoA
-
Bos taurus
1.3.8.8
0.24
-
cis,cis,cis,cis-4,7,10,13-hexadecatetraenoyl-CoA
-
Bos taurus
1.3.8.8
0.25
-
4-cis-decenoyl-CoA
-
Bos taurus
1.3.8.8
0.29
-
stearoyl-CoA
-
Bos taurus
1.3.8.8
0.32
-
4-cis,7-cis-decadienoyl-CoA
-
Bos taurus
1.3.8.8
0.32
-
6-cis-decenoyl-CoA
-
Bos taurus
1.3.8.8
0.38
-
tetradecanoyl-CoA
-
Bos taurus
1.3.8.8
0.55
-
hexanoyl-CoA
-
Bos taurus
1.3.8.8
0.78
-
palmitoyl-CoA
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.8.8
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.8.1
40300
-
4 * 40300, SDS-PAGE
Bos taurus
1.3.8.1
188000
-
gel filtration
Bos taurus
1.3.8.7
43300
-
4 * 43300, SDS-PAGE
Bos taurus
1.3.8.7
205000
-
native gel electrophoresis in polyacrylamide gradient
Bos taurus
1.3.8.8
172000
-
gel electrophoresis
Bos taurus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.3.8.1
Bos taurus
-
-
-
1.3.8.7
Bos taurus
-
-
-
1.3.8.8
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.3.8.1
-
Bos taurus
1.3.8.7
-
Bos taurus
1.3.8.8
40-70% ammonium sulfate precipitation, Sephadex G-150, DEAE-cellulose, preparative electrophoresis
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.8.1
liver
-
Bos taurus
-
1.3.8.7
liver
-
Bos taurus
-
1.3.8.8
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.8.1
1.42
-
-
Bos taurus
1.3.8.1
17.9
-
-
Bos taurus
1.3.8.8
32.5
-
purified enzyme, decanoyl-CoA
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptors phenazine methosulfate or Meldola' s Blue (i.e. 8-dimethylamino-2,3-benzophenoxazinium chloride)
391275
Bos taurus
2-butenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.1
butyryl-CoA + electron acceptor
terminal electron acceptor iodonitrotetrazolium chloride
391275
Bos taurus
2-butenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.1
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
52% of activity with butyryl-CoA
391275
Bos taurus
2-hexenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.1
octanoyl-CoA + Meldola's Blue + iodonitrotetrazolium chloride
14.4% of activity with butyryl-CoA
391275
Bos taurus
2-octenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
4-cis,7-cis-decadienoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans,4-cis,7cis-decatrienoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
4-cis-decenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans,4-cis-decadienoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
4-decynoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
trans-2-ene-4-decynoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
5-cis-decenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans,5-cis-decadienoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
6-cis-decenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans,6-cis-decadienoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
acyl-CoA + acceptor
2-p-iodophenyl-3-p-nitrophenyl-5-phenyl-2H-tetrazolium chloride as final electron acceptor
391275
Bos taurus
2,3-dehydroacyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
all-cis-4,7,10,13-hexadecatetraenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans-all-cis-4,7,10,13-hexadecapentaenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
butanoyl-CoA + acceptor
-
391275
Bos taurus
crotonyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
decanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
electron acceptor: Meldola's Blue and iodonitrotetrazoliumchloride, general and long-chain acyl-CoA dehydrogenase
391275
Bos taurus
2-trans-decenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
dodecanoyl-CoA + tetrazolium salt
-
391275
Bos taurus
trans-dodec-2-enoyl-CoA + reduced tetrazolium salt
-
-
-
r
1.3.8.7
hexadecanoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans-hexadecenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
hexanoyl-CoA + tetrazolium salt
-
391275
Bos taurus
trans-hex-2-enoyl-CoA + reduced tetrazolium salt
-
-
-
r
1.3.8.7
tetradecanoyl-CoA + tetrazolium salt
-
391275
Bos taurus
tetradecenoyl-CoA + reduced tetrazolium salt
-
-
-
r
1.3.8.8
4-cis-7-cis-decadienoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-4,7-cis,cis-decatrienoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
4-cis-decenoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-4-cis-decadienoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
5-cis-decenoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-5-cis-decadienoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
6-cis-decenoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-6-cis-decadienoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
cis,cis,cis,cis-4,7,10,13-hexadecatetraenoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-4,7,10,13-cis,cis,cis,cis-hexadecapentaenoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
decanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-decenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
dodecanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-dodecenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
hexanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
(2E)-2-hexenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
octanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
(2E)-2-octenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
palmitoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-hexadecenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
stearoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-octadecenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
tetradecanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-tetradecenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.8.1
tetramer
4 * 40300, SDS-PAGE
Bos taurus
1.3.8.7
tetramer
4 * 43300, SDS-PAGE
Bos taurus
1.3.8.8
tetramer
4 * 38000-45200, SDS-PAGE
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.8.1
0.009
-
butyryl-CoA
-
Bos taurus
1.3.8.1
0.02
-
hexanoyl-CoA
-
Bos taurus
1.3.8.1
0.032
-
octanoyl-CoA
-
Bos taurus
1.3.8.7
0.002
-
all-cis-4,7,10,13-hexadecatetraenoyl-CoA
-
Bos taurus
1.3.8.7
0.0021
-
6-cis-decenoyl-CoA
-
Bos taurus
1.3.8.7
0.0023
-
4-cis,7-cis-decadienoyl-CoA
-
Bos taurus
1.3.8.7
0.0029
-
decanoyl-CoA
-
Bos taurus
1.3.8.7
0.0029
-
4-decynoyl-CoA
-
Bos taurus
1.3.8.7
0.003
-
5-cis-decenoyl-CoA
-
Bos taurus
1.3.8.7
0.0032
-
octanoyl-CoA
-
Bos taurus
1.3.8.7
0.004
-
hexanoyl-CoA
-
Bos taurus
1.3.8.7
0.004
-
4-cis-decenoyl-CoA
-
Bos taurus
1.3.8.7
0.0043
-
dodecanoyl-CoA
-
Bos taurus
1.3.8.7
0.005
-
hexanoyl-CoA
-
Bos taurus
1.3.8.7
0.0057
-
tetradecanoyl-CoA
-
Bos taurus
1.3.8.7
0.0084
-
butanoyl-CoA
-
Bos taurus
1.3.8.8
0.13
-
decanoyl-CoA
-
Bos taurus
1.3.8.8
0.2
-
dodecanoyl-CoA
-
Bos taurus
1.3.8.8
0.2
-
5-cis-decenoyl-CoA
-
Bos taurus
1.3.8.8
0.23
-
octanoyl-CoA
-
Bos taurus
1.3.8.8
0.24
-
cis,cis,cis,cis-4,7,10,13-hexadecatetraenoyl-CoA
-
Bos taurus
1.3.8.8
0.25
-
4-cis-decenoyl-CoA
-
Bos taurus
1.3.8.8
0.29
-
stearoyl-CoA
-
Bos taurus
1.3.8.8
0.32
-
4-cis,7-cis-decadienoyl-CoA
-
Bos taurus
1.3.8.8
0.32
-
6-cis-decenoyl-CoA
-
Bos taurus
1.3.8.8
0.38
-
tetradecanoyl-CoA
-
Bos taurus
1.3.8.8
0.55
-
hexanoyl-CoA
-
Bos taurus
1.3.8.8
0.78
-
palmitoyl-CoA
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.8.8
mitochondrion
-
Bos taurus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.8.1
40300
-
4 * 40300, SDS-PAGE
Bos taurus
1.3.8.1
188000
-
gel filtration
Bos taurus
1.3.8.7
43300
-
4 * 43300, SDS-PAGE
Bos taurus
1.3.8.7
205000
-
native gel electrophoresis in polyacrylamide gradient
Bos taurus
1.3.8.8
172000
-
gel electrophoresis
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.8.1
-
Bos taurus
1.3.8.7
-
Bos taurus
1.3.8.8
40-70% ammonium sulfate precipitation, Sephadex G-150, DEAE-cellulose, preparative electrophoresis
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.8.1
liver
-
Bos taurus
-
1.3.8.7
liver
-
Bos taurus
-
1.3.8.8
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.8.1
1.42
-
-
Bos taurus
1.3.8.1
17.9
-
-
Bos taurus
1.3.8.8
32.5
-
purified enzyme, decanoyl-CoA
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptors phenazine methosulfate or Meldola' s Blue (i.e. 8-dimethylamino-2,3-benzophenoxazinium chloride)
391275
Bos taurus
2-butenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.1
butyryl-CoA + electron acceptor
terminal electron acceptor iodonitrotetrazolium chloride
391275
Bos taurus
2-butenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.1
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
52% of activity with butyryl-CoA
391275
Bos taurus
2-hexenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.1
octanoyl-CoA + Meldola's Blue + iodonitrotetrazolium chloride
14.4% of activity with butyryl-CoA
391275
Bos taurus
2-octenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
4-cis,7-cis-decadienoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans,4-cis,7cis-decatrienoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
4-cis-decenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans,4-cis-decadienoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
4-decynoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
trans-2-ene-4-decynoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
5-cis-decenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans,5-cis-decadienoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
6-cis-decenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans,6-cis-decadienoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
acyl-CoA + acceptor
2-p-iodophenyl-3-p-nitrophenyl-5-phenyl-2H-tetrazolium chloride as final electron acceptor
391275
Bos taurus
2,3-dehydroacyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
all-cis-4,7,10,13-hexadecatetraenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans-all-cis-4,7,10,13-hexadecapentaenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
butanoyl-CoA + acceptor
-
391275
Bos taurus
crotonyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
decanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
electron acceptor: Meldola's Blue and iodonitrotetrazoliumchloride, general and long-chain acyl-CoA dehydrogenase
391275
Bos taurus
2-trans-decenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
dodecanoyl-CoA + tetrazolium salt
-
391275
Bos taurus
trans-dodec-2-enoyl-CoA + reduced tetrazolium salt
-
-
-
r
1.3.8.7
hexadecanoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride
cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase
391275
Bos taurus
2-trans-hexadecenoyl-CoA + reduced acceptor
-
391275
Bos taurus
?
1.3.8.7
hexanoyl-CoA + tetrazolium salt
-
391275
Bos taurus
trans-hex-2-enoyl-CoA + reduced tetrazolium salt
-
-
-
r
1.3.8.7
tetradecanoyl-CoA + tetrazolium salt
-
391275
Bos taurus
tetradecenoyl-CoA + reduced tetrazolium salt
-
-
-
r
1.3.8.8
4-cis-7-cis-decadienoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-4,7-cis,cis-decatrienoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
4-cis-decenoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-4-cis-decadienoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
5-cis-decenoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-5-cis-decadienoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
6-cis-decenoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-6-cis-decadienoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
cis,cis,cis,cis-4,7,10,13-hexadecatetraenoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-trans-4,7,10,13-cis,cis,cis,cis-hexadecapentaenoyl-CoA + reduced meldola blue
-
391275
Bos taurus
?
1.3.8.8
decanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-decenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
dodecanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-dodecenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
hexanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
(2E)-2-hexenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
octanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
(2E)-2-octenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
palmitoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-hexadecenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
stearoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-octadecenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
1.3.8.8
tetradecanoyl-CoA + Meldola's Blue
-
391275
Bos taurus
2-tetradecenoyl-CoA + reduced meldola blue
trans-isomer
391275
Bos taurus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.8.1
tetramer
4 * 40300, SDS-PAGE
Bos taurus
1.3.8.7
tetramer
4 * 43300, SDS-PAGE
Bos taurus
1.3.8.8
tetramer
4 * 38000-45200, SDS-PAGE
Bos taurus