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Literature summary extracted from

  • Knecht, W.; Bergjohann, U.; Gonski, S.; Kirschbaum, B.; Loffler, M.
    Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme (1996), Eur. J. Biochem., 240, 292-301.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide A77 1726, active metabolite of leflumonide Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.004
-
 dihydroorotate cosubstrate ubiquinone-50 Homo sapiens
1.3.5.2 0.009
-
 dihydroorotate cosubstrate decylubiquinone Homo sapiens
1.3.5.2 0.01
-
 ubiquinone-50 cosubstrate dihydroorotate Homo sapiens
1.3.5.2 0.014
-
 decylubiquinone cosubstrate dihydroorotate Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.2 42000
-
 gel filtration, truncated recombinant protein Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Homo sapiens
-
 truncated recombinant protein
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + ubiquinone-50
-
 Homo sapiens orotate + ubiquinol-50
-
 r
1.3.5.2 dihydroorotate + acceptor acceptor: ubiquinone Homo sapiens orotate + reduced acceptor
-
 ?
1.3.5.2 L-dihydroorotate + decylubiquinone
-
 Homo sapiens orotate + reduced decylubiquinone
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.2 monomer SDS-PAGE, truncated recombinant protein Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.5.2 0.0002 0.0009 2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide competitive to dihydroorotate Homo sapiens
1.3.5.2 0.001
-
 2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide non-competitive to decyclubiquinone Homo sapiens