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Literature summary extracted from

  • Yoshinaga, T.
    Purification and properties of coproporphyrinogen III oxidase from bovine liver (1997), Methods Enzymol., 281, 355-367.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.3.3 2-mercaptoethanol slight activation Bos taurus
1.3.3.3 DTT slight activation Bos taurus
1.3.3.3 lecithin 2fold activation Mus musculus
1.3.3.3 Non-ionic detergents 2fold activation Mus musculus
1.3.3.3 Non-ionic detergents 2-4fold activation Bos taurus
1.3.3.3 Phospholipids 2-4fold activation Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.3 expression in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.3 H158A complete loss of activity, decreased stability, no copper content Mus musculus

General Stability

EC Number General Stability Organism
1.3.3.3 tends to aggregate during prolonged storage at 4°C Mus musculus
1.3.3.3 tends to aggregate during prolonged storage at 4°C Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.3 additional information decreased activity at high ionic strength Bos taurus
1.3.3.3 SDS inhibitory Bos taurus
1.3.3.3 sodium deoxycholate inhibitory Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.3 0.047
-
 Coproporphyrinogen-III
-
 Mus musculus
1.3.3.3 0.048
-
 Coproporphyrinogen-III
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.3 mitochondrion
-
 Mus musculus 5739
-
1.3.3.3 mitochondrion located within intermembrane space Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.3 Cu2+ 0.06 atom per enzyme Bos taurus
1.3.3.3 Cu2+ 1.05 atoms per enzyme Mus musculus
1.3.3.3 Fe2+ 0.07-0.14 atom per enzyme Bos taurus
1.3.3.3 Fe2+ trace amounts Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.3 37000
-
 x * 37000, SDS-PAGE Mus musculus
1.3.3.3 37000
-
 x * 37000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+ Bos taurus
-
 protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+ Mus musculus 6th enzyme in heme biosynthetic pathway protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.3 Bos taurus
-
-
-
1.3.3.3 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.3 to homogeneity, chromatography steps Bos taurus
1.3.3.3 to homogeneity, recombinant enzyme Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.3 liver
-
 Mus musculus
-
1.3.3.3 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.3 0.3
-
-
 Mus musculus
1.3.3.3 0.7
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+
-
 Bos taurus protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+ sixths enzyme in heme biosynthetic pathway Mus musculus protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+ 6th enzyme in heme biosynthetic pathway Mus musculus protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.3 ? x * 37000, SDS-PAGE Mus musculus
1.3.3.3 ? x * 37000, SDS-PAGE Bos taurus