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Literature summary extracted from
- Biosynthetic dihydroorotate dehydrogenase from Lactobacillus bulgaricus (1971), J. Bacteriol., 105, 1015-1027.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.3.98.1 | 20% loss of activity after 1 h exposure to room light | Lactobacillus delbrueckii subsp. bulgaricus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.98.1 | additional information | visible light is inactivating | Lactobacillus delbrueckii subsp. bulgaricus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.98.1 | soluble | - |
Lactobacillus delbrueckii subsp. bulgaricus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.98.1 | (NH4)2SO4 | enhances enzymatic activity up to a concentration of 0.0014 mM | Lactobacillus delbrueckii subsp. bulgaricus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.98.1 | 53000 | - |
gel filtration, crude fraction | Lactobacillus delbrueckii subsp. bulgaricus |
| 1.3.98.1 | 57000 | - |
gel filtration | Lactobacillus delbrueckii subsp. bulgaricus |
| 1.3.98.1 | 57000 | - |
purified fraction | Lactobacillus delbrueckii subsp. bulgaricus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.98.1 | (S)-dihydroorotate + O2 | Lactobacillus delbrueckii subsp. bulgaricus | pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme | orotate + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.98.1 | Lactobacillus delbrueckii subsp. bulgaricus | - |
09, ATCC 13866 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.98.1 | ion-exchange, gel filtration, ammonium sulfate precipitation | Lactobacillus delbrueckii subsp. bulgaricus |
| 1.3.98.1 | preparative gel electrophoresis | Lactobacillus delbrueckii subsp. bulgaricus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.3.98.1 | (S)-dihydroorotate + fumarate = orotate + succinate | light sensitivity seems to result in a conformational change and a partial loss of enzymatic activity | Lactobacillus delbrueckii subsp. bulgaricus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.98.1 | 117.5 | - |
with ferricyanide as acceptor | Lactobacillus delbrueckii subsp. bulgaricus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.3.98.1 | -20°C, progressive enzyme decay | Lactobacillus delbrueckii subsp. bulgaricus |
| 1.3.98.1 | 4°C, purified enzyme, stable up to two years | Lactobacillus delbrueckii subsp. bulgaricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.98.1 | (S)-dihydroorotate + ferricyanide | - |
Lactobacillus delbrueckii subsp. bulgaricus | orotate + ferrocyanide | - |
? | |
| 1.3.98.1 | (S)-dihydroorotate + O2 | redox dyes, 2,6-dichlorophenolindophenol, ferricyanide are most active electron acceptors, oxygen and cytochrome c are much slower | Lactobacillus delbrueckii subsp. bulgaricus | orotate + H2O2 | - |
? | |
| 1.3.98.1 | (S)-dihydroorotate + O2 | pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme | Lactobacillus delbrueckii subsp. bulgaricus | orotate + H2O2 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.98.1 | 23 | 25 | assay at | Lactobacillus delbrueckii subsp. bulgaricus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.98.1 | 2.7 | - |
S-dihydroorotate | with oxygen | Lactobacillus delbrueckii subsp. bulgaricus | |
| 1.3.98.1 | 157 | - |
ferricyanide | - |
Lactobacillus delbrueckii subsp. bulgaricus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.98.1 | 7.6 | 7.8 | - |
Lactobacillus delbrueckii subsp. bulgaricus |
| 1.3.98.1 | 7.7 | - |
with ferricyanide as electron acceptor | Lactobacillus delbrueckii subsp. bulgaricus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.98.1 | FMN | 1 mol per 55000 g of protein | Lactobacillus delbrueckii subsp. bulgaricus | |
| 1.3.98.1 | FMN | flavoprotein, enzyme contains 1 FMN as prosthetic group | Lactobacillus delbrueckii subsp. bulgaricus | |
| 1.3.98.1 | additional information | no FAD as prosthetic group | Lactobacillus delbrueckii subsp. bulgaricus |
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Release 2023.1 (January 2023)
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