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Literature summary extracted from

  • Nikaido, H.; Moriishi, M.; Mishima, H.K.; Kitamura, S.; Tatsumi, K.
    15-Ketoprostaglandin delta 13-reductase activity in bovine ocular tissues (1993), Curr. Eye Res., 12, 17-22.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.48 dicoumarol 0.1 mM, 86% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2 Bos taurus
1.3.1.48 Disulfiram 0.1 mM, 61% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2 Bos taurus
1.3.1.48 indomethacin 0.1 mM, 97% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2 Bos taurus
1.3.1.48 quercitrin 0.1 mM, 76% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.48 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.48 55000
-
 gel filtration Bos taurus
1.3.1.48 57000
-
 1 * 57000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.48 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.48
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.48 ciliary body
-
 Bos taurus
-
1.3.1.48 cornea
-
 Bos taurus
-
1.3.1.48 iris
-
 Bos taurus
-
1.3.1.48 retina retinal pigment epithelium choroid Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.48 0.00026
-
 enzyme activity in cornea, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus
1.3.1.48 0.000389
-
 enzyme activity in retina, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus
1.3.1.48 0.00085
-
 enzyme activity in retinal pigment epithelium, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus
1.3.1.48 0.000945
-
 enzyme purified from iris-ciliary cytosol Bos taurus
1.3.1.48 0.0012
-
 enzyme activity in ciliary body, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus
1.3.1.48 0.00145
-
 enzyme activity in iris, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.3.1.48 -80°C, NADH-linked activity is lost overnight Bos taurus
1.3.1.48 0°C, NADPH-linked activity is lost after a few hours Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.48 11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
 Bos taurus 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.48 monomer 1 * 57000, SDS-PAGE Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.48 NAD(P)H enzyme from iris-ciliary body prefers NADPH Bos taurus