Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Prasad, M.R.; Nagi, M.N.; Cook, L.; Cinti, D.L.
    Kinetic evidence for two separate trans-2-enoyl CoA reductases in rat hepatic microsomes: NADPH-specific short chain- and NAD(P)H-dependent long chain-reductase (1983), Biochem. Biophys. Res. Commun., 113, 659-665.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.8 microsome
-
 Rattus norvegicus
-
-
1.3.1.38 microsome
-
 Rattus norvegicus
-
-
1.3.1.44 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.8 Rattus norvegicus
-
 separation in NADPH-specific short-chain enoyl-CoA reductase and NAD(P)H-dependent long-chain enoyl-CoA reductase
-
1.3.1.8 Rattus norvegicus
-
 no information in the literature concerning reduction of cis-2-enoyl-CoA substrates, thus classification of rat enzyme according to EC 1.3.1.8 or EC 1.3.1.38 impossible
-
1.3.1.38 Rattus norvegicus
-
 no information in the literature concerning reduction of cis-2-enoyl-CoA substrates, thus classification of rat enzyme according to EC 1.3.1.8 or EC 1.3.1.38 not yet possible
-
1.3.1.44 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.8 liver
-
 Rattus norvegicus
-
1.3.1.38 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.38 0.005
-
 substrate crotonyl-CoA Rattus norvegicus
1.3.1.38 0.05
-
 substrate trans-hexenoyl-CoA Rattus norvegicus
1.3.1.44 0.005
-
 substrate trans-2-octenoyl-CoA with NADH Rattus norvegicus
1.3.1.44 0.02
-
 substrate trans-2-hexadecenoyl-CoA with NADH Rattus norvegicus
1.3.1.44 0.027
-
 substrate trans-2-hexadecenoyl-CoA with NADPH Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.8 2-trans-hexadecenoyl-CoA + NADPH
-
 Rattus norvegicus hexadecanoyl CoA + NADP+
-
 ?
1.3.1.8 2-trans-hexenoyl-CoA + NAD(P)H
-
 Rattus norvegicus hexanoyl-CoA + NADP+
-
 ?
1.3.1.8 2-trans-octenoyl-CoA + NAD(P)H
-
 Rattus norvegicus octanoyl-CoA + NADP+
-
 ?
1.3.1.8 crotonyl-CoA + NAD(P)H
-
 Rattus norvegicus butanoyl-CoA + NADP+
-
 ?
1.3.1.8 additional information NADPH specific trans-enoyl-CoA reductases isolated from rat liver microsomes and mitochondria impossible to classify according to EC 1.3.1.8 or EC 1.3.1.38 because no cis-isomers of 2-enoyl-CoA have been tested as substrates Rattus norvegicus ?
-
 ?
1.3.1.38 crotonyl-CoA + NADPH
-
 Rattus norvegicus butanoyl-CoA + NADP+
-
 ?
1.3.1.38 additional information NADPH-specific trans-enoyl-CoA reductases from rat liver miochondria and microsomes impossible to classify according to EC 1.3.1.8 or EC 1.3.1.38 because no cis-isomers of 2-enoyl-CoA have been tested as substrates Rattus norvegicus ?
-
 ?
1.3.1.38 trans-2-hexenoyl-CoA + NADPH
-
 Rattus norvegicus hexanoyl-CoA + NADP+
-
 ?
1.3.1.44 additional information long-chain substrates preferred Rattus norvegicus ?
-
 ?
1.3.1.44 trans-2-hexadecenoyl-CoA + NADH
-
 Rattus norvegicus hexadecanoyl-CoA + NAD+
-
 ?
1.3.1.44 trans-2-octenoyl-CoA + NADH
-
 Rattus norvegicus octanoyl-CoA + NAD+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.38 37
-
 assay at Rattus norvegicus
1.3.1.44 37
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.8 NADPH inactive with NADH Rattus norvegicus
1.3.1.38 NADPH
-
 Rattus norvegicus
1.3.1.44 NADH preferred cofactor with substrates of higher chain length Rattus norvegicus
1.3.1.44 NADPH
-
 Rattus norvegicus