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Literature summary extracted from

  • Coulter, C.V.; Gerrard, J.A.; Kraunsoe, J.A.E.; Pratt, A.J.
    Escherichia coli dihydrodipicolinate synthase and dihydrodipicolinate reductase: kinetic and inhibition studies of two putative herbicide targets (1999), Pestic. Sci., 55, 887-895.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.17.1.8 agriculture potential herbicide target, leads for herbicide development are inhibitors: dipicolinic acid, isophthalic acid, and DELTA3-tetrahydroisophthalic acid Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.8 dapB gene, overexpression from plasmid in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.8 (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate
-
 Escherichia coli
1.17.1.8 DELTA3-tetrahydroisophthalic acid moderate inhibition Escherichia coli
1.17.1.8 dipicolinate reversible, competitive versus substrate (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate Escherichia coli
1.17.1.8 isophthalic acid reversible, competitive versus substrate 2,3-dihydrodipicolinate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.8 0.0066
-
 NADH
-
 Escherichia coli
1.17.1.8 0.0165
-
 NADPH
-
 Escherichia coli
1.17.1.8 0.529
-
 (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.8 2,3-dihydrodipicolinate + NAD(P)H Escherichia coli
-
 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.8 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.8 overexpression in Escherichia coli from plasmid Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.8 135
-
 purified enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.8 (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + NADPH + H+
-
 Escherichia coli ?
-
 r
1.17.1.8 2,3-dihydrodipicolinate + NAD(P)H
-
 Escherichia coli 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.8 ? ? * 28757-29000, SDS-PAGE and sequence determination Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.17.1.8 DHDPR
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.8 NADH
-
 Escherichia coli
1.17.1.8 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.17.1.8 0.435
-
 (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate
-
 Escherichia coli
1.17.1.8 5.5
-
 isophthalic acid
-
 Escherichia coli