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Literature summary extracted from

  • Furuebisu, M.; Deguchi, S.; Okuda, K.
    Identification of cortisone 5beta-reductase as DELTA4-3-ketosteroid 5beta-reductase (1987), Biochim. Biophys. Acta, 912, 110-114.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.3 p-chloromercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.0015
-
 cortisone
-
 Rattus norvegicus
1.3.1.3 0.0022
-
 4-androstene-3,17-dione
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.3 4-androstene-3,17-dione + NADPH Rattus norvegicus
-
 5beta-androstan-3,17-dione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Rattus norvegicus
-
 cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein
-
1.3.1.3 Rattus norvegicus
-
 cortisone 5beta-reductase and reduction of androstenedione (3-oxo-5beta-steroid:NADP+ DELTA4-oxidoreductase activity, EC 1.3.1.23) catalyzed by the same catalytic site of a single protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.3
-
 Rattus norvegicus
1.3.1.3 cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.3 0.034
-
 purified enzyme, substrate cortisone Rattus norvegicus
1.3.1.3 0.0341
-
-
 Rattus norvegicus
1.3.1.3 0.0354
-
 purified enzyme, substrate androstenedione Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 4-androstene-3,17-dione + NADPH
-
 Rattus norvegicus 5beta-androstan-3,17-dione + NADP+
-
 ?
1.3.1.3 androst-4-en-3,17-dione + NADPH
-
 Rattus norvegicus 5beta-androstan-3,17-dione + NADP+
-
 ?
1.3.1.3 cortisone + NADPH + H+
-
 Rattus norvegicus 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.3 More cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.3 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.3 50
-
 60 s, 50% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.3 7.4
-
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.3 NADPH
-
 Rattus norvegicus
1.3.1.3 NADPH absolutely required Rattus norvegicus